Detection of SHV-1 β-lactamase in Pseudomonas aeruginosa strains by genetic methods
| Autor: | Mohamed Bejaoui, Abdeladhim Ben Abdeladhim, A. Ben Hassen, S. Kalai Blagui, W. Achour |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Microbial Sensitivity Tests Biology medicine.disease_cause beta-Lactamases DNA sequencing Microbiology Enterobacteriaceae Klebsiella Drug Resistance Bacterial Genotype medicine Pulsed-field gel electrophoresis Humans DNA Primers Gel electrophoresis Pseudomonas aeruginosa Isoelectric focusing DNA–DNA hybridization Nucleic acid sequence General Medicine Penicillinase biochemical phenomena metabolism and nutrition bacterial infections and mycoses Drug Resistance Multiple Electrophoresis Gel Pulsed-Field Isoelectric Focusing |
| Zdroj: | Pathologie Biologie. 57:e73-e75 |
| ISSN: | 0369-8114 |
| Popis: | Twelve multidrug-resistant Pseudomonas aeruginosa (MDRPA) isolates were recovered over a period of two years in the National Bone Marrow Transplant Centre of Tunisia. MDRPA isolates were isolated from seven patients and from three environmental samples. Isoelectric focusing revealed pIs of 8.2, 5.5 and 7.6 in all MDRPA isolates. These strains produced the OXA-18 extended spectrum beta-lactamase and an SHV type beta-lactamase as shown by screening PCR analysis. DNA hybridization confirmed this inference, detecting bla(SHV) gene in these isolates. Pulsed-field gel electrophoresis (PFGE) defined one predominant genomic group; group A (seven isolates) and four different genotypes containing one to two isolates. Clonally related isolates were recovered from three patients and from two washbasins. Sequencing DNA of cluster representative strains identified the classical bla(SHV-1) gene. For these strains, the nucleotide sequence of the structural bla(SHV-1) gene was nearly identical to those previously described. Such enzyme has not been reported from P. aeruginosa. This is the first report of the SHV-1 penicillinase in epidemic P. aeruginosa strain. |
| Databáze: | OpenAIRE |
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