Improvement of the effect of hepatocyte isograft in the Gunn rat by cotransplantation of islets of Langerhans

Autor: Claude Pierrette Le Coultre, Bernard Genin, Philippe Morel, Jacques Birraux, Laura Rubbia-Brandt, Andereggen E
Rok vydání: 1999
Předmět:
Blood Glucose
medicine.medical_specialty
Orthotopic liver transplantation
Cell Transplantation
Isograft
Rats
Gunn
Islets of Langerhans Transplantation
Liver/cytology
Spleen
ddc:616.07
digestive system
Statistics
Nonparametric
Hepatocyte transplantation
Animal model
Internal medicine
medicine
Animals
Cell Transplantation/physiology
Hyperbilirubinemia
geography
Analysis of Variance
ddc:618
Hyperbilirubinemia/blood/surgery
geography.geographical_feature_category
ddc:617
business.industry
General Medicine
Blood Glucose/analysis
Islet
Gunn rat
Liver Transplantation
Rats
Islets of Langerhans Transplantation/physiology
Disease Models
Animal
Liver Transplantation/physiology
Spleen/surgery
medicine.anatomical_structure
Endocrinology
Liver
Hepatocyte
Pediatrics
Perinatology and Child Health
Surgery
business
Zdroj: Journal of Pediatric Surgery, Vol. 34, No 2 (1999) pp. 321-4
ISSN: 0022-3468
Popis: Purpose: Hepatocyte transplantation (HcTX) has been investigated for many years as an alternative therapy to orthotopic liver transplantation to treat hepatic congenital enzymatic deficiency disease. The animal model most used is the Gunn rat, which presents a hyperbilirubinemia caused by the lack of uridine-diphosphate-glucuronyl-transferase. Some investigators have clearly described a hepatotrophic effect mediated by islets of Langerhans (IL) when transplanted with hepatocytes (Hc). In this study, the functional effect of cotransplanted IL on hepatocytes (co-HcTX) in Gunn rats in an isograft model is assessed. Methods: Two groups are compared: group 1 (n = 6), HcTX to group 2 (n = 6), co-HcTX. Cells isolated by enzymatic digestion are transplanted directly into the splenic parenchyma. Blood samples are taken regularly until day 100 to measure the unconjugated bilirubin (UB). Histological examination of the spleen is performed at the end of the experiment. Results: Both groups show a significant decrease of the UB: group 1, 47%; group 2, 65%. The decrease is statistically more pronounced with co-HcTX. The histological analysis shows a trophic effect of the IL on the grafted hepatocytes in the co-HcTX group. Conclusions: The HcTX and the co-HcTX correct partially the hyperbilirubinemia of the Gunn rat. A functional assessment has been performed to evaluate the effect of cotransplanted IL on HcTX.
Databáze: OpenAIRE
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