Effects of hypo- and hyperoxia on transcription levels of five stress genes and the glutathione system in liver of Atlantic cod Gadus morhua
| Autor: | Torstein Kristensen, Bjørn Olav Rosseland, Knut Erik Tollefsen, Rune Waagbø, Pål A. Olsvik, H. Toften |
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| Rok vydání: | 2006 |
| Předmět: |
Fish Proteins
Antioxidant Transcription Genetic Physiology medicine.medical_treatment Aquatic Science medicine.disease_cause Andrology chemistry.chemical_compound medicine Metallothionein Gadus Animals Phospholipid-hydroperoxide glutathione peroxidase Molecular Biology Ecology Evolution Behavior and Systematics Hyperoxia biology Glutathione Disulfide Gene Expression Regulation Developmental Water Glutathione biology.organism_classification Oxygen Oxidative Stress chemistry Biochemistry Gadus morhua Liver Catalase Insect Science biology.protein Animal Science and Zoology medicine.symptom Oxidative stress |
| Zdroj: | The Journal of experimental biology. 209(Pt 15) |
| ISSN: | 0022-0949 |
| Popis: | SUMMARY The transcript levels of three genes coding for antioxidants, Cu/Zn superoxide dismutase (SOD), catalase and phospholipid hydroperoxide glutathione peroxidase (GSH-Px), and those of two stress proteins,metallothionein (MT) and CYP1A, were examined with real-time quantitative (q)RT-PCR in hepatic tissue of Atlantic cod exposed to 46% (hypoxia), 76%(normoxia) and 145% (hyperoxia) O2 saturation (tank outlet). To evaluate the oxidative stress state, the levels of total glutathione (tGSH),reduced glutathione (GSH) and oxidized glutathione (GSSG) and subsequently the oxidative stress index (OSI), were determined in the same tissue samples. The transcript level of GSH-Px was significantly upregulated in fish exposed to hyperoxia, and significantly downregulated in fish exposed to hypoxia,compared to the normoxia group. Significant downregulation was also found for SOD and CYP1A transcriptional levels in fish exposed to hypoxia. The transcript levels of catalase and MT did not change in liver of cod exposed to suboptimal oxygen levels. No significant differences were seen between the groups for tGSH, GSH, GSSG or OSI. Prolonged exposure to unfavourable oxygen saturation levels did not alter the OSI, indicating that the antioxidant glutathione system is maintained at an unchanged level in liver of the examined cod. |
| Databáze: | OpenAIRE |
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