Interaction of selected platinum(II) complexes containing roscovitine-based CDK inhibitors as ligands with human liver microsomal cytochrome P450
| Autor: | Eva Anzenbacherova, Pavel Štarha, David Milde, Monika Harvanova, Martina Michalova, Zdenek Travnicek, Vlastimil Mašek |
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| Rok vydání: | 2014 |
| Předmět: |
cdk inhibition
Stereochemistry lcsh:Medicine Platinum Compounds Hydroxylation Ligands General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound roscovitine derivatives platinum(ii) complexes medicine Cytochrome P-450 CYP3A Humans Demethylation CYP3A4 biology lcsh:R Bufuralol Cytochrome P450 Coumarin Biochemistry chemistry cytochrome p450 Chlorzoxazone Microsome biology.protein Microsomes Liver Oxidation-Reduction medicine.drug |
| Zdroj: | Biomedical Papers, Vol 159, Iss 3, Pp 382-387 (2015) |
| ISSN: | 1804-7521 |
| Popis: | Background: We studied the interaction of oxaliplatin derivatives involving cytotoxic adenine-based cyclin-dependent kinase inhibitors, with human liver microsomal cytochrome P450. Methods and Results: The activities of 9 human liver microsomal CYP forms (CYPs 1A2, 7-ethoxyresorufin O-deethylation; 2A6, coumarin 7-hydroxylation; 2B6, 7-ethoxy-4-(trifluoromethyl) coumarin O-deethylation; 2C8, luciferin-6´ methyl ether demethylation; 2C9, diclofenac 4´-hydroxylation, 6´-deoxyluciferin hydroxylation; 2C19, (S)-mephenytoin 4´-hydroxylation; 2D6, bufuralol 1´-hydroxylation, 2E1, chlorzoxazone 6-hydroxylation; 3A4, testosterone 6β-hydroxylation, luciferin-6´ benzyl ether debenzylation) were tested using HPLC, fluorescence and luminescence product detection. At 100 µM platinum(II) oxalato complex concentration, CYP inhibition was in general 25%-50%, except for the CYP3A4 form which showed roughly twice the inhibition (72%-95%). At low complex concentration (10 µM), the difference in inhibition of CYP3A4 and other forms was even more pronounced. Dixon and Lineweaver-Burk plots indicated a partially noncompetitive mechanism of CYP3A4 inhibition. Conclusions: The tested complexes significantly inhibit human liver microsomal CYP3A4 activity even at clinically relevant concentrations. This could be a serious drawback for the use of these compounds in clinical practice. |
| Databáze: | OpenAIRE |
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