Oxidative stress and inflammatory markers in patients with COVID-19: Potential role of RAGE, HMGB1, GFAP and COX-2 in disease severity
| Autor: | Fabiolla Rocha Santos, Passos, Luana, Heimfarth, Brenda Souza, Monteiro, Cristiane Bani, Corrêa, Tatiana Rodrigues de, Moura, Adriano Antunes de Souza, Araújo, Paulo Ricardo, Martins-Filho, Lucindo José, Quintans-Júnior, Jullyana de Souza Siqueira, Quintans |
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| Rok vydání: | 2022 |
| Předmět: |
Adult
Male Adolescent Receptor for Advanced Glycation End Products Immunology Severity of Illness Index Article Young Adult Glial Fibrillary Acidic Protein Humans Immunology and Allergy HMGB1 Protein Child Aged Aged 80 and over Inflammation HMGB1 Pharmacology neurological dysfunction SARS-CoV-2 GFAP COVID-19 Middle Aged COX-2 Healthy Volunteers RAGE Up-Regulation Oxidative Stress Cyclooxygenase 2 Case-Control Studies Female Biomarkers |
| Zdroj: | International Immunopharmacology |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2021.108502 |
| Popis: | Background SARS-CoV-2 infection can lead to the abnormal induction of cytokines and a dysregulated hyperinflammatory state that is implicated in disease severity and risk of death. There are several molecules present in blood associated with immune cellular response, inflammation, and oxidative stress that could be used as severity markers in respiratory viral infections such as COVID-19. However, there is a lack of clinical studies evaluating the role of oxidative stress-related molecules including glial fibrillary acidic protein (GFAP), the receptor for advanced glycation end products (RAGE), high mobility group box-1 protein (HMGB1) and cyclo-oxygenase-2 (COX-2) in COVID-19 pathogenesis. Aim To evaluate the role of oxidative stress-related molecules in COVID-19. Method An observational study with 93 Brazilian participants from September 2020 to April 2021, comprising 23 patients with COVID-19 admitted to intensive care unit (ICU), 19 outpatients with COVID-19 with mild to moderate symptoms, 17 individuals reporting a COVID-19 history, and 34 healthy controls. Blood samples were taken from all participants and western blot assay was used to determine the RAGE, HMGB1, GFAP, and COX-2 immunocontent. Results We found that GFAP levels were higher in patients with severe or critical COVID-19 compared to outpatients (p = 0.030) and controls (p < 0.001). A significant increase in immunocontents of RAGE (p < 0.001) and HMGB1 (p < 0.001) were also found among patients admitted to the ICU compared to healthy controls, as well as an overexpression of the inducible COX-2 (p < 0.001). In addition, we found a moderate to strong correlation between RAGE, GFAP and HMGB1 proteins. Conclusion SARS-CoV-2 infection induces the upregulation of GFAP, RAGE, HMGB1, and COX-2 in patients with the most severe forms of COVID-19. |
| Databáze: | OpenAIRE |
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