Differences in amino acid and lipid metabolism distinguish Crohn's from idiopathic/cryptoglandular perianal fistulas by tissue metabonomic profiling and may offer clues to underlying pathogenesis
| Autor: | Kapil Sahnan, R. K. S. Phillips, Elaine Holmes, Nik S. Ding, Phil Tozer, Janindra Warusavitarne, Magali Sarafian, Ailsa Hart, Samuel O. Adegbola, Omar Faiz |
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| Rok vydání: | 2020 |
| Předmět: |
Anal fistula
medicine.medical_specialty Hepatology business.industry Fistula Gastroenterology Lipid metabolism Pilot Projects Disease medicine.disease Lipid Metabolism Pathophysiology Pathogenesis Treatment Outcome Crohn Disease Internal medicine medicine Metabolome Humans Metabolomics Rectal Fistula Microbiome Amino Acids business |
| Zdroj: | European journal of gastroenterologyhepatology. 33(12) |
| ISSN: | 1473-5687 |
| Popis: | INTRODUCTION Few studies have investigated perianal fistula etiopathogenesis, and although the cryptoglandular theory is widely accepted in idiopathic cases, in Crohn's disease, it is thought to involve the interplay between microbiological, immunological and genetic factors. A pilot study was conducted to assess for metabolic variations in Crohn's perianal fistula tissue that might differ from that of idiopathic (cryptoglandular) perianal fistula tissue as a comparator. The goal was to identify any potential biomarkers of disease, which may improve the understanding of pathogenesis. AIMS AND METHODS Fistula tract biopsies were obtained from 30 patients with idiopathic perianal fistula and 20 patients with Crohn's anal fistula. Two different assays were used in an ultra-high-performance liquid chromatography system coupled with a mass spectrometric detector to achieve broad metabolome coverage. Univariate and multivariate statistical data analyses were used to identify differentiating metabolic features corresponding to the perianal fistula phenotype (i.e. Crohn's disease vs. idiopathic). RESULTS Significant orthogonal partial least squares discriminant analysis predictive models (validated with cross-validated-analysis of variance P value |
| Databáze: | OpenAIRE |
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