Taming the Huntington’s Disease Proteome: What Have We Learned?
| Autor: | Connor Seeley, Kimberly B. Kegel-Gleason |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
0301 basic medicine Huntingtin Proteome Systems biology interactome Review Computational biology Biology Interactome Mass Spectrometry Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Huntington's disease medicine Animals Humans Amino Acids Differential expression Biological sciences Brain Chemistry Huntingtin Protein medicine.disease Huntington Disease 030104 developmental biology Neurology (clinical) HTT 030217 neurology & neurosurgery Huntington’s disease |
| Zdroj: | Journal of Huntington's Disease |
| ISSN: | 1879-6400 1879-6397 |
| DOI: | 10.3233/jhd-200465 |
| Popis: | Mass spectrometry (MS) is a physical technique used to identify specific chemicals and molecules by precise analysis of their mass and charge; this technology has been adapted for biological sciences applications. Investigators have used MS to identify differential expressions of proteins in Huntington’s disease (HD), to discover Huntingtin (HTT) interacting proteins and to analyze HTT proteoforms. Using systems biology and computational approaches, data from MS screens have been leveraged to find differentially expressed pathways. This review summarizes the data from most of the MS studies done in the HD field in the last 20 years and compares it to the protein data reported before the use of MS technology. The MS results validate early findings in the field such as differential expression of PDE10a and DARPP-32 and identify new changes. We offer a perspective on the MS approach in HD, particularly for identification of disease pathways, the challenges in interpreting data across different studies, and its application to protein studies moving forward. |
| Databáze: | OpenAIRE |
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