Nasal Epithelial Cells as Surrogates for Bronchial Epithelial Cells in Airway Inflammation Studies
| Autor: | J. Graham Douglas, Garry Michael Walsh, Richard J. Brooker, Peter Joseph Benedict Helms, Morgan Graeme Blaylock, C. M. McDougall |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Receptor expression Clinical Biochemistry Cell Bronchi Nose Biology Cytokeratin Microscopy Electron Transmission Cell surface receptor medicine Humans Child Cell Shape Molecular Biology Cells Cultured Aged Aged 80 and over Inflammation CD44 Infant Epithelial Cells Articles Cell Biology Middle Aged respiratory system Epithelium Nasal Mucosa medicine.anatomical_structure Child Preschool biology.protein Cytokines Respiratory epithelium Female Intracellular |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 39:560-568 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/rcmb.2007-0325oc |
| Popis: | The nose is an attractive source of airway epithelial cells, particularly in populations in which bronchoscopy may not be possible. However, substituting nasal cells for bronchial epithelial cells in the study of airway inflammation depends upon comparability of responses, and evidence for this is lacking. Our objective was to determine whether nasal epithelial cell inflammatory mediator release and receptor expression reflect those of bronchial epithelial cells. Paired cultures of undifferentiated nasal and bronchial epithelial cells were obtained from brushings from 35 subjects, including 5 children. Cells were subject to morphologic and immunocytochemical assessment. Mediator release from resting and cytokine-stimulated cell monolayers was determined, as was cell surface receptor expression. Nasal and bronchial cells had identical epithelial morphology and uniform expression of cytokeratin 19. There were no differences in constitutive expression of CD44, intercellular adhesion molecule-1, alphavbeta3, and alphavbeta5. Despite significantly higher constitutive release of IL-8, IL-6, RANTES (regulated on activation, normal T cell expressed and secreted), and matrix metalloproteinase (MMP)-9 from nasal compared with bronchial cells, the increments in release of all studied mediators in response to stimulation with IL-1beta and TNF-alpha were similar, and there were significant positive correlations between nasal and bronchial cell secretion of IL-6, RANTES, vascular endothelial growth factor, monocyte chemoattractant protein-1, MMP-9, and tissue inhibitor of metalloproteinase-1. Despite differences in absolute mediator levels, the responses of nasal and bronchial epithelial cells to cytokine stimulation were similar, expression of relevant surface receptors was comparable, and there were significant correlations between nasal and bronchial cell mediator release. Therefore, nasal epithelial cultures constitute an accessible surrogate for studying lower airway inflammation. |
| Databáze: | OpenAIRE |
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