The histone deacetylase inhibitor trichostatin A alters microRNA expression profiles in apoptosis-resistant breast cancer cells
| Autor: | Bridgette M. Collins-Burow, Steven Elliott, Meng Li, Elizabeth C. Martin, Lyndsay V. Rhodes, Ashley M. Nitschke, Matthew E. Burow, Seung Yoon Nam, Jennifer L. Driver, H. Chris Segar, Kenneth P. Nephew |
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| Rok vydání: | 2011 |
| Předmět: |
Cancer Research
medicine.drug_class Gene Expression Apoptosis Breast Neoplasms Biology Hydroxamic Acids Article Cell Line Tumor microRNA medicine Humans skin and connective tissue diseases Oligonucleotide Array Sequence Analysis Gene Expression Profiling Histone deacetylase inhibitor Cancer General Medicine Cell cycle medicine.disease Histone Deacetylase Inhibitors MicroRNAs Trichostatin A Oncology MCF-7 Drug Resistance Neoplasm Cancer research Female Histone deacetylase Epigenetic therapy medicine.drug |
| Zdroj: | Oncology Reports. |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2011.1488 |
| Popis: | The development of drug resistance represents a major complication in the effective treatment of breast cancer. Epigenetic therapy, through the use of histone deacetylase inhibitors (HDACi) or demethylation agents, is an emerging area of therapeutic targeting in a number of ontological entities, particularly in the setting of aggressive therapy-resistant disease. Using the well-described HDAC inhibitor trichostatin A (TSA) we demonstrate the suppression of in vitro clonogenicity in the previously described apoptosis-resistant MCF-7TN-R breast carcinoma cell line. Additionally, recent work has demonstrated that these agents can alter the expression profile of microRNA signatures in malignant cells. Using an unbiased microRNA microarray analysis, changes in miRNA expression of MCF-7TN-R cells treated with TSA for 24 h were analyzed. We observed significant up-regulation of 22 miRNAs and down-regulation of 10 miRNAs in response to TSA treatment. Our results demonstrate that the HDACi, TSA, exerts anticancer activity in the apoptosis-resistant MCF-7TN-R breast carcinoma cell line. This activity is correlated with TSA alteration of microRNA expression profiles indicative of a less aggressive phenotype. |
| Databáze: | OpenAIRE |
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