Biocompatibility of magnetic nanoparticles coating with polycations using A549 cells
| Autor: | Ramil Fakhrullin, Elvira Rozhina, Svetlana Batasheva, Farida Akhatova, Anna Danilushkina, Artem Rozhin |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Biocompatibility Coprecipitation Cell Survival Nanoparticle Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Allylamine Nanomaterials 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Humans Polyethyleneimine Tissue Distribution Magnetite Nanoparticles Polyethylenimine Chemistry General Medicine Polyelectrolytes Polyelectrolyte 030104 developmental biology A549 Cells Magnetic nanoparticles Nanoparticles Biotechnology Nuclear chemistry |
| Zdroj: | Journal of biotechnology. 325 |
| ISSN: | 1873-4863 |
| Popis: | Fe3O4 nanoparticles were obtained by chemical coprecipitation of iron chloride and sodium hydroxide. The morphology and sizes of the obtained nanoparticles were characterized using laser Doppler velocimetry, transmission electron and atomic force microscopy. Then the nanoparticles were stabilized by three polycations (polyethylenimine (PEI), poly(allylamine hydrochloride) (PAH), poly(diallyldimethylammonium chloride) (PDADMAC)) to increase their biocompatibility. The cytotoxicity of the obtained polymer-stabilized nanoparticles was studied using a human lung carcinoma cell line (A549). The biodistribution of nanoparticles stabilized by polycations in human lung carcinoma cells was analyzed by transmission electron microscopy, and the toxicity of nanomaterials was evaluated using toxicity tests and flow cytometry. As a result, the most biocompatible nanoparticle-biopolymer complex was identified. PAH stabilized magnetic nanoparticles demonstrated the best biocompatibility, and the PEI-magnetic nanoparticle complex was the most toxic. |
| Databáze: | OpenAIRE |
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