GEL FILTRATION OF OESTROGEN CONJUGATES IN BOVINE URINE

Autor:	S. A. Miklosi, P. J. McCOSKER
Rok vydání:	1967
Předmět:	Carbon Isotopes Chromatography medicine.medical_specialty Estrogens Conjugated (USP) Estradiol Estrone Chemistry Endocrinology Diabetes and Metabolism Size-exclusion chromatography Urine Endocrinology Internal medicine Chromatography Gel Methods medicine Animals Cattle Steroids Conjugate
Zdroj:	Journal of Endocrinology. 39:361-368
ISSN:	1479-6805 0022-0795
DOI:	10.1677/joe.0.0390361
Popis:	SUMMARY A chromatographic method for the purification of oestrogen conjugates in bovine urine is described. The sample is made 1·0 m in NaCl and applied to a 45 × 6·2 cm. column of Sephadex G-25. Elution with water results in a constant and reproducible retardation and simultaneous purification of the urinary conjugates. Authentic oestrogen conjugates and radioactive urinary metabolites were used in the experiments. The extent of purification and values for recovery are presented and the use of activated charcoal for preliminary decolorization is described.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72312ec44346e6267931efa077852ffd https://doi.org/10.1677/joe.0.0390361 Zobrazit plný text záznamu