The transcriptome of hand eczema assessed by tape stripping
Autor: | Marie-Louise Kampmann, Jeppe Dyrberg Andersen, Jacob P. Thyssen, Julie Sølberg, Stine B Jacobsen, Niels Morling, Jeanne D. Johansen, Anna S Quaade, Thomas Litman |
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Rok vydání: | 2021 |
Předmět: |
Adult
Male medicine.medical_specialty Whole Transcriptome Sequencing Total rna Down-Regulation Hand Dermatoses contact dermatitis Dermatology Dermatitis Atopic Specimen Handling Diagnosis Differential Transcriptome transcriptomics Exome Sequencing medicine Humans Immunology and Allergy Surgical Tape Aged Skin integumentary system Receptor EphA1 business.industry tape stripping Interleukin-8 Atopic dermatitis Middle Aged medicine.disease atopic hand eczema Hand eczema Dermatitis Allergic Contact Irritant contact dermatitis Dermatitis Irritant Female Molecular Profile business Contact dermatitis Biomarkers subtypes of hand eczema |
Zdroj: | Sølberg, J B K, Quaade, A S, Jacobsen, S B, Andersen, J D, Kampmann, M L, Morling, N, Litman, T, Thyssen, J P & Johansen, J D 2022, ' The transcriptome of hand eczema assessed by tape stripping ', Contact Dermatitis, vol. 86, no. 2, pp. 71-79 . https://doi.org/10.1111/cod.14015 |
ISSN: | 1600-0536 0105-1873 |
Popis: | Background No biomarkers have been identified that can classify subtypes of hand eczema (HE). Although skin biopsies represent the golden standard for investigations of the skin, the invasive technique is not favorable when investigating skin from sensitive areas. Recent advances in the use of skin tape strips for molecular investigations enable non-invasive investigations of HE. Objective By using whole transcriptome sequencing (WTS), the molecular profile of HE according to different localizations on the hands, etiologies, and clinical/morphological subtypes was investigated. Methods Thirty adult, Danish HE patients, 12 with and 18 without concurrent atopic dermatitis (AD), as well as 16 controls were included. Tape strip samples were collected from lesional, non-lesional, and healthy skin. Total RNA was extracted, and WTS was performed. Results The largest molecular difference of HE patients with and without AD was found in non-lesional skin areas and included a downregulation of CXCL8 for HE patients without AD. Differences between allergic- and irritant contact dermatitis included promising epidermal biomarkers such as EPHA1. Conclusion Skin tape strip samples could be used to assess the gene expression profile of HE on different localizations of the hands. The skin tape strip method identified new molecular markers that showed promising result for the identification of HE subtypes. This article is protected by copyright. All rights reserved. |
Databáze: | OpenAIRE |
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