Mutational analysis of the 3′-terminal extra-cistronic region of poliovirus RNA: secondary structure is not the only requirement for minus strand RNA replication
| Autor: | R. Perez Bercoff, Mauro Bucci, Anna Marta Degener, Alessandra Pierangeli, P. Pagnotti |
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| Rok vydání: | 1995 |
| Předmět: |
viruses
Molecular Sequence Data Biophysics RNA-dependent RNA polymerase Biology Transfection Biochemistry Template interaction Cell Line Structural Biology Transcription (biology) Secondary structure Genetics Animals Deoxyribonucleases Type II Site-Specific 3′-Untranslated region Molecular Biology Base Sequence Picornavirus Intron RNA Cell Biology Mutational analysis Non-coding RNA Virology Molecular biology Antisense RNA Poliovirus Genes Mutagenesis RNA editing Nucleic Acid Conformation RNA Viral Transcription Small nuclear RNA |
| Zdroj: | FEBS Letters. 374:327-332 |
| ISSN: | 0014-5793 |
| Popis: | A series of mutations were introduced in the 3′-terminal untranslated region (3′-UTR) of full-length infectious poliovirus cDNA clones, and following transfection of COS-1 cells the ability of these constructs to generate viable viral particles and/or to support viral RNA synthesis was assayed. Substitution of the 3′-UTR of poliovirus RNA with the equivalent sequences of HAV RNA abrogated viral RNA replication, whereas the introduction of extended ‘foreign’ sequences between the open reading frame and the 3′-UTR was well tolated. Point mutation that either destabilized the stem-and-loop structure or altered the sequence of the loop in domain ‘Y’ (nomenclature as per Pilipenko et al., [Nucleic Acids Res. 20 (1992) 1739–1745]) abolished both the infectivity and viral RNA synthesis. These were not restored by compensatory mutation that reconstructed the native secondary structure of this domain, suggesting that the secondary/tertiary folding of the 3′-UTR is not the only determinant for template recognition at initiation of RNA synthesis, but rather that a specific primary sequence is indeed required. |
| Databáze: | OpenAIRE |
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