A longitudinal study of various crevicular fluid components as markers of periodontal disease activity
| Autor: | Patrick Brochut, G. Cimasoni, Nicolas Roehrich, Bertrand Dubrez, Catherine Giannopoulou, Koichi Nakashima, Elene Andersen |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Male medicine.medical_specialty Neutrophils Osteocalcin Acrylic Resins Sensitivity and Specificity Dinoprostone Leukocyte Count Internal medicine Periodontal Attachment Loss medicine Humans alpha-Macroglobulins Aspartate Aminotransferases Collagenases Longitudinal Studies Prostaglandin E2 Periodontitis Pancreatic elastase Glucuronidase biology Pancreatic Elastase Chemistry Elastase Discriminant Analysis Gingival Crevicular Fluid Alkaline Phosphatase Macroglobulin Endocrinology Clinical attachment loss alpha 1-Antitrypsin Immunology Collagenase biology.protein Alkaline phosphatase Periodontics Female Stents Biomarkers medicine.drug Follow-Up Studies |
| Zdroj: | Journal of clinical periodontology. 23(9) |
| ISSN: | 0303-6979 |
| Popis: | In order to examine the relationship of possible crevicular biochemical parameters to attachment loss (ALOSS), 330 sites from 8 untreated adult patients were monitored longitudinally at 3-month intervals, for up to 1 year. Attachment levels were measured with a force-sensing probe and an acrylic stent in duplicates at each study point. Crevicular samples were collected and used for the determination of the following 11 markers: number of polymorphonuclear leukocytes (PMNs), prostaglandin E2 (PGE2), osteocalcin (OC), alkaline phosphatase (ALP), collagenase (COL), beta-glucuronidase (BG), antigenic and functional elastase (AEL and FEL), alpha-1 antitrypsin (a1AT), alpha-2 macroglobulin (a2M) and aspartate aminotransferase (AST). 10 sites with ALOSS of > or = 1.5 mm per 3 months (active sites) and 43 sites with negligible changes (inactive sites) were identified. Total amounts of ALP, BG and COL were found to be significantly higher in active as compared to inactive sites, prior to significant ALOSS, without any significant differences in crevicular fluid volume and clinical indices. When biochemical parameters were expressed as ratios to the number of PMNs, PGE2/ PMNs was significantly elevated in active sites. The capacity of such individual parameters to distinguish between active and inactive sites was limited. However, linear discriminant analysis using total amounts of PGE2, COL, ALP, a2M, OC and AEL showed more significant diagnostic values (sensitivity: 80%, specificity: 91%). These findings suggest that the combination of several biochemical parameters in crevicular fluid could give more information to predict future clinical ALOSS. |
| Databáze: | OpenAIRE |
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