An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor
| Autor: | Abbas Maqbool, Tolga O. Bozkurt, Yasin F. Dagdas, Jan Sklenar, Pooja Pandey, Joe Win, Nadra Tabassum, Richard K. Hughes, Sophien Kamoun, Benjamin Petre, Neftaly Cruz-Mireles, Khaoula Belhaj, Mark J. Banfield, Angela Chaparro-Garcia, Frank L.H. Menke, Kim Findlay |
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| Přispěvatelé: | The Sainsbury Laboratory [Norwich] (TSL), Department of Biological Chemistry, John Innes Centre, Norwich, UK, John Innes Centre [Norwich]-University of East Anglia [Norwich] (UEA), Department of Life Sciences, Imperial College London, London, United Kingdom, Department of Cell and Developmental Biology, John Innes Centre [Norwich], Biotechnology and Biological Sciences Research Council (BBSRC) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Life Sciences & Biomedicine - Other Topics [SDV]Life Sciences [q-bio] Plant Biology Plasma protein binding Arabidopsis DISEASE RESISTANCE Biology (General) Plant Proteins 2. Zero hunger Fungal protein Microbiology and Infectious Disease biology Effector General Neuroscience food and beverages General Medicine ARABIDOPSIS Cell biology RXLR EFFECTORS Phytophthora infestans Medicine Life Sciences & Biomedicine Irish potato famine Research Article effectors Programmed cell death autophagy QH301-705.5 Science infectious disease late blight disease IMMUNITY General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Botany Nicotiana benthamiana TRAFFICKING SELECTIVE AUTOPHAGY PLANT Biology Secretory pathway Plant Diseases Solanum tuberosum Science & Technology General Immunology and Microbiology Autophagy microbiology fungi SECRETORY PATHWAY biology.organism_classification 030104 developmental biology CELL-DEATH Other RESPONSES |
| Zdroj: | eLife eLife, eLife Sciences Publication, 2016, 5, ⟨10.7554/eLife.10856⟩ eLife, Vol 5 (2016) |
| ISSN: | 2050-084X |
| Popis: | Plants use autophagy to safeguard against infectious diseases. However, how plant pathogens interfere with autophagy-related processes is unknown. Here, we show that PexRD54, an effector from the Irish potato famine pathogen Phytophthora infestans, binds host autophagy protein ATG8CL to stimulate autophagosome formation. PexRD54 depletes the autophagy cargo receptor Joka2 out of ATG8CL complexes and interferes with Joka2's positive effect on pathogen defense. Thus, a plant pathogen effector has evolved to antagonize a host autophagy cargo receptor to counteract host defenses. DOI: http://dx.doi.org/10.7554/eLife.10856.001 eLife digest Plants and other living organisms can survive stress and starvation by digesting and recycling parts of their own cells. This process is known as autophagy and it involves engulfing cellular material inside spherical structures called autophagosomes, before delivering it to sites in the cell where digestive enzymes can break the material down. A form of autophagy, known as selective autophagy, can specifically degrade toxic substances such as disease-causing microbes. Selective autophagy works through proteins called autophagy cargo receptors that define which molecules are targeted for degradation. However, it was not clear whether autophagy protects plants from infections, or how much disease-causing microbes interfere with this process for their own benefit. The microbe that causes late blight of potatoes (called Phytophthora infestans) is infamous for triggering widespread famines in Ireland in the 19th century. This disease-causing microbe continues to pose a serious threat to food security today, and parasitizes plant tissues by releasing proteins called effectors that enter the plant’s cells to subvert the plant’s physiology and counteract its defenses. Dagdas, Belhaj et al. now report that an effector from P. infestans, called PexRD54, can bind to autophagy-related protein from potato, called ATG8CL, and stimulate the formation of autophagosomes. Further experiments revealed that the PexRD54 effector could outcompete a plant autophagy cargo receptor that would otherwise bind to ATG8CL. This plant cargo receptor contributes to the plant’s defences, and by preventing it from interacting with ATG8CL, PexRD54 makes the plant more susceptible to infection by P. infestans. These findings show that the PexRD54 effector has evolved to interact with an autophagy-related protein to counteract the plant’s defences. Dagdas, Belhaj et al. suggest that PexRD54 might do this by activating autophagy to selectively eliminate some of the molecules that the plant use to defend itself. Furthermore, P. infestans might also benefit from the nutrients that are released when cellular material is broken down via autophagy. Future work could test these two hypotheses and explore whether other effectors from disease-causing microbes work in a similar way. DOI: http://dx.doi.org/10.7554/eLife.10856.002 |
| Databáze: | OpenAIRE |
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