Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats
| Autor: | Mengmeng Wang, Fen Li, Xiangyu Xi, Peng-Bo Wen, Xin-Yi Shi, Qing-Hua Liu, Xue-Song Xiong, Xiao Zhang, Eng Guan Chua, Liang Wang, Jia-Wei Tang, Zhanzhong Liu, Zhao Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Intestinal microbiota 030209 endocrinology & metabolism Pharmacology High-performance liquid chromatography Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Other systems of medicine 0302 clinical medicine HFD/STZ treatment Diabetes mellitus Type 2 diabetes mellitus medicine Animals Ethanol Glycogen Plant Extracts Type 2 Diabetes Mellitus medicine.disease Streptozotocin 16 s rDNA Gastrointestinal Microbiome Liver Glycogen Plant Leaves 030104 developmental biology Diabetes Mellitus Type 2 Complementary and alternative medicine chemistry Dysbiosis Ethanol extract of mulberry leaves Morus Dyslipidemia RZ201-999 Research Article medicine.drug |
| Zdroj: | BMC Complementary Medicine and Therapies, Vol 21, Iss 1, Pp 1-15 (2021) BMC Complementary Medicine and Therapies |
| ISSN: | 2662-7671 |
| Popis: | Background Mulberry leaf as a traditional Chinese medicine is able to treat obesity, diabetes, and dyslipidemia. It is well known that diabetes leads to intestinal microbiota dysbiosis. It is also recently discovered that liver glycogen structure is impaired in diabetic animals. Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure. Methods In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day). Composition of intestinal microbiota was analyzed via metagenomics by sequencing the V3-V4 region of 16S rDNAs. Liver glycogen structure was characterized through size exclusion chromatography (SEC). Both Student’s t-test and Tukey’s test were used for statistical analysis. Results A group of type 2 diabetic rat models were successfully established. Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions. In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves. Conclusions This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats. These finding can be helpful in discovering the novel therapeutic targets with the help of further investigations. |
| Databáze: | OpenAIRE |
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