Iridovirus in the root weevil Diaprepes abbreviatus
| Autor: | S. L. Lapointe, C. J. Funk, D. S. Achor, Wayne B. Hunter, X. H. Sinisterra |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Citrus Sexual transmission food.ingredient Transovarial transmission Iridovirus weevil Virus food sexually transmitted Animals transovarial Insect virus lcsh:QH301-705.5 Ovum Beetle biology Transmission (medicine) Weevil Articles insect virus General Medicine biology.organism_classification Virology biological insecticide entomopathogen Infectious Disease Transmission Vertical mating Diaprepes abbreviatus injection lcsh:Biology (General) Virus Diseases Larva Insect Science ingestion Weevils Female Chilo iridescent virus IIV6 |
| Zdroj: | Journal of Insect Science, Vol 3, p 9 (2003) Journal of Insect Science Europe PubMed Central |
| ISSN: | 1536-2442 0970-3837 |
| DOI: | 10.1673/031.003.0901 |
| Popis: | Invertebrate iridescent virus 6 (IIV6) was evaluated for mode of transmission and ability to cause infection in the root weevil, Diaprepes abbreviatus (L.). This is the first evidence of IIV6 infection in D. abbreviatus, which caused both patent and sub-lethal covert infections in both larvae and adults. Adults and larvae were successfully infected with IIV6 by puncture, injection and per os. Transmission of IIV6 was demonstrated between infected and healthy individuals regardless of gender. Virus was detected in egg masses produced by virus-infected females suggesting IIV6 is transmitted transovarially. Virus particles were observed in the cytoplasm of weevil cells, and were shown to infect fat bodies, muscle, and nerve tissues, as visualized using transmission electron microscopy. Patent infections resulted in death of individuals within 3 to 4 days post infection. Individuals with covert infections tested positive for virus infection on day 7 by polymerase chain reaction analysis. Sequencing of PCR amplicons confirmed virus infection. Discovery of new pathogens against root weevils may provide new management tools for development of control strategies based on induced epizootics. This is the first report of a virus infecting D. abbreviatus. |
| Databáze: | OpenAIRE |
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