The Chromatin Remodeler ISW1 Is a Quality Control Factor that Surveys Nuclear mRNP Biogenesis
| Autor: | Anna Babour, Qingtang Shen, Julien Dos-Santos, Struan Murray, Alexandre Gay, Drice Challal, Milo Fasken, Benoît Palancade, Anita Corbett, Domenico Libri, Jane Mellor, Catherine Dargemont |
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| Přispěvatelé: | Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins [SDV]Life Sciences [q-bio] RNA polymerase II Saccharomyces cerevisiae [SDV.BC]Life Sciences [q-bio]/Cellular Biology Exosomes General Biochemistry Genetics and Molecular Biology Chromatin remodeling 03 medical and health sciences 0302 clinical medicine Transcription (biology) [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Nuclear export signal ComputingMilieux_MISCELLANEOUS Genetics Adenosine Triphosphatases Messenger RNA [SDV.GEN]Life Sciences [q-bio]/Genetics biology Exosome Multienzyme Ribonuclease Complex Chromatin binding Extra View Chromatin Assembly and Disassembly Chromatin Cell biology DNA-Binding Proteins 030104 developmental biology Ribonucleoproteins Multiprotein Complexes biology.protein RNA Polymerase II 030217 neurology & neurosurgery Biogenesis |
| Zdroj: | Cell Cell, Elsevier, 2016, 167 (5), pp.1201-1214.e15. ⟨10.1016/j.cell.2016.10.048⟩ |
| ISSN: | 0092-8674 1097-4172 |
| DOI: | 10.1016/j.cell.2016.10.048⟩ |
| Popis: | Chromatin dynamics play an essential role in regulating DNA transaction processes, but it is unclear whether transcription-associated chromatin modifications control the mRNA ribonucleoparticles (mRNPs) pipeline from synthesis to nuclear exit. Here, we identify the yeast ISW1 chromatin remodeling complex as an unanticipated mRNP nuclear export surveillance factor that retains export-incompetent transcripts near their transcription site. This tethering activity of ISW1 requires chromatin binding and is independent of nucleosome sliding activity or changes in RNA polymerase II processivity. Combination of in vivo UV-crosslinking and genome-wide RNA immunoprecipitation assays show that Isw1 and its cofactors interact directly with premature mRNPs. Our results highlight that the concerted action of Isw1 and the nuclear exosome ensures accurate surveillance mechanism that proofreads the efficiency of mRNA biogenesis. |
| Databáze: | OpenAIRE |
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