Nudeotide sequence of the chicken 5-aminolevulinate synthase gene
| Autor: | Brian K. May, W.H. Elliott, Adrienne R. Day, Peter L. Wigley, Gopesh Srivastava, Deborah J. Maguire, Iain A. Borthwick |
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| Rok vydání: | 1986 |
| Předmět: |
Regulation of gene expression
Base Sequence Genetic Linkage Structural gene Nucleic acid sequence Chromosome Mapping Mitochondria Liver Chick Embryo DNA Restriction Enzymes Biology Molecular biology Primer extension Exon Gene Expression Regulation Genes Biochemistry Genetics Histone H2B Animals Amino Acid Sequence Promoter Regions Genetic Chickens Peptide sequence Gene 5-Aminolevulinate Synthetase |
| Zdroj: | Nucleic Acids Research. 14:1379-1391 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/14.3.1379 |
| Popis: | 5-Aminolevulinate synthase, the first and rate-controlling enzyme of heme biosynthesis, is regulated in the liver by the end-product heme. To study this negative control mechanism, we have isolated the chicken gene for ALA-synthase and determined the nucleotide sequence. The structural gene is 6.9 kb long and contains 10 exons. The transcriptional start site for ALA-synthase was determined by primer extension analysis. A fragment of 291 bp from the 5' flanking region including 34 bp of the first exon shows promoter activity when introduced upstream of a chicken histone H2B gene and injected into the nuclei of Xenopus laevis oocytes. |
| Databáze: | OpenAIRE |
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