Implications of Blood Contamination for Assessment of Local Cellular Immunity in the Endocervix

Autor:	Susan Cu-Uvin, Cinnie Chou, Deborah J. Anderson, Peggy A. Crowley-Nowick, Alison J. Quayle, Ruth Tuomala, Joseph A. Politch, Meha Shah, Ann Duerr
Rok vydání:	2004
Předmět:	Immunity Cellular Cellular immunity T-Lymphocytes Blood contamination T cell Immunology Cervix Uteri Biology Flow Cytometry Infectious Diseases medicine.anatomical_structure Minimal effect Virology medicine Humans Female Local immunity Endocervix
Zdroj:	AIDS Research and Human Retroviruses. 20:543-546
ISSN:	1931-8405 0889-2229
DOI:	10.1089/088922204323087796
Popis:	Cervical cytobrushes provide a tool to sample endocervical T cells for assessment of local immunity. However, most previous studies in HIV-seropositive women have excluded samples containing blood and hence have analyzed selected populations of patients. As determined by multiple-parameter flow cytometric analysis of T lymphocytes from two sequential cytobrushes and concurrently collected blood samples, this study found a minimal effect of blood contamination on cervical T cell phenotypic parameters in normal women. The consequences of blood in endocervical samples will ultimately depend on the design and objective of each study, but these data suggest studies could be more inclusive and should not automatically discard samples that contain red blood cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72253dc89751e1bcb0ca08121162a59b https://doi.org/10.1089/088922204323087796 Zobrazit plný text záznamu