Chemical synthesis of native S‐palmitoylated membrane proteins through removable‐backbone‐modification‐assisted Ser/Thr ligation

Autor: Changlin Tian, Ji-Shen Zheng, Ying Li, Nadège Jamin, Dong-Liang Huang, Yong Zheng, Jun Liang, Cédric Montigny, Thomas Barbot, Christine Jaxel, Veronica Beswick, Xiu-Xiu Cao, Min Xue
Přispěvatelé: Chinese Academy of Sciences [Beijing] (CAS), Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China [Hefei] (USTC), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Protéines et Systèmes Membranaires (LPSM), Département Biochimie, Biophysique et Biologie Structurale (B3S), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Threonine
congenital
hereditary
and neonatal diseases and abnormalities
Serine/threonine ligation
Proteolipids
[SDV]Life Sciences [q-bio]
education
Palmitates
Muscle Proteins
010402 general chemistry
Chemical synthesis
01 natural sciences
Catalysis
Ion Channels
Membrane proteins
Protein biosynthesis
Serine
Animals
Amino Acid Sequence
skin and connective tissue diseases
Ion channel
Solid-Phase Synthesis Techniques
Chemical protein synthesis
Chemistry
010405 organic chemistry
Aminobutyrates
food and beverages
Removable backbone modification
General Chemistry
General Medicine
0104 chemical sciences
Sarcolipin
body regions
S-palmitoylation
Membrane protein
Biochemistry
Solubility
Rabbits
Chemical protein synthesis * Serine/threonine ligation * Removable backbone modification * S-palmitoylation * Membrane proteins
Ligation
Peptides
Zdroj: Angewandte Chemie International Edition
Angewandte Chemie International Edition, 2019, 59 (13), pp.5178-5184. ⟨10.1002/anie.201914836⟩
Angewandte Chemie International Edition, Wiley-VCH Verlag, 2019, ⟨10.1002/anie.201914836⟩
Angewandte Chemie International Edition, Wiley-VCH Verlag, 2019, 59 (13), pp.5178-5184. ⟨10.1002/anie.201914836⟩
ISSN: 1433-7851
1521-3773
DOI: 10.1002/anie.201914836⟩
Popis: International audience; Preparation of native S-palmitoylated (S-palm) membrane proteins represents one of the unsolved challenges in chemical protein synthesis. Herein, we reported the first chemical synthesis of S-palm membrane proteins by reversible backbone modification assisted Ser/Thr Ligation (RBM$^{GABA}$ -assisted STL). This method involves two critical steps: (1) Synthesis of S-palm peptides by a new γ-aminobutyric acid group-based RBM (RBM$^{GABA}$) strategy, (2) Ligation of the S-palm RBM-modified peptides to give the desired S-palm product by STL. The utility of RBM$^{GABA}$ -assisted STL method was demonstrated by the synthesis of rabbit S-palm sarcolipin (SLN) and the S-palm matrix-2 (M2) ion channel. The synthesis of S-palm membrane proteins highlights the importance of developing non-NCL methods for chemical protein synthesis.
Databáze: OpenAIRE
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