High-throughput method for the evaluation of esterase activity in soils
| Autor: | Hiroko Kitamoto, Shun Tsuboi, Kimiko Yamamoto-Tamura, Takumi Tanaka |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) chemistry.chemical_classification Chromatography Bacteria Esterases Substrate (chemistry) Valerate complex mixtures Microbiology Esterase High-Throughput Screening Assays Cold Temperature 03 medical and health sciences Soil 030104 developmental biology chemistry Soil water Molecular Biology Soil Microbiology Enzyme Assays |
| Zdroj: | SC30201804040010 NARO成果DBa 著者版依頼中／Applying for the author's permission. |
| ISSN: | 1872-8359 |
| Popis: | We describe a method to quickly evaluate soil esterase activity using p‑nitrophenyl valerate as the substrate. Unwanted coloration of the control samples was suppressed by cooling. Esterase activity can be evaluated using arbitrary amounts of soil. Sample dispensation was simplified and the number of examinations per soil sample reduced. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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