Porcine PPARGC1A (peroxisome proliferative activated receptor gamma coactivator 1A): coding sequence, genomic organization, polymorphisms and mapping
| Autor: | H. Barthenschlager, Gary A. Rohrer, Martine Yerle, A. Van Zeveren, François Piumi, Luc Peelman, M. Van Poucke, Marc Mattheeuws, K. Jacobs |
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| Rok vydání: | 2004 |
| Předmět: |
Swine
Peroxisome Proliferative Activated Receptor Gamma Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Coactivator Genetics Coding region Animals Humans Molecular Biology Genetics (clinical) In Situ Hybridization Fluorescence Genomic organization DNA Primers Polymorphism Genetic Base Sequence Chromosome Mapping Introns Adipose Tissue Organ Specificity Trans-Activators PPARGC1A Body Temperature Regulation Transcription Factors |
| Zdroj: | Cytogenetic and genome research. 112(1-2) |
| ISSN: | 1424-859X |
| Popis: | We report here the characterisation of porcine PPARGC1A. Primers based on human PPARGC1A were used to isolate two porcine BAC clones. Porcine coding sequences of PPARGC1A were sequenced together with the splice site regions and the 5′ and 3′ regions. Using direct sequencing nine SNPs were found. Allele frequencies were determined in unrelated animals of five different pig breeds. In the MARC Meishan-White Composite resource population, the polymorphism in exon 9 was significantly associated with leaf fat weight. PPARGC1A has been mapped by FISH to SSC8p21. A (CA)n microsatellite (SGU0001) has been localised near marker SWR1101 on chromosome 8 by RH mapping and at the same position as marker KS195 (32.5 cM) by linkage mapping. The AseI (nt857, Asn/Asn489) polymorphism in exon 8 was used to perform linkage analysis in the Hohenheim pedigrees and located the gene in the same genomic region. Transcription of the gene was detected in adipose, muscle, kidney, liver, brain, heart and adrenal gland tissues, which is in agreement with the function of PPARGC1A in adaptive thermogenesis. |
| Databáze: | OpenAIRE |
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