Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features

Autor: Luisa Helena Monteiro de Miranda, Tassia Cristina Bello de Vasconcelos, Noëlle Doyen, Vinícius Silva Belo, Sávio Freire Bruno, Monique Paiva de Campos, Jean-Marc Cavaillon, Maria de Fátima Madeira, Fabiano Borges Figueiredo
Přispěvatelé: Instituto de Computação [Niteroi-Rio de Janeiro] (IC-UFF), Universidade Federal Fluminense [Rio de Janeiro] (UFF), Cytokines et Inflammation, Institut Pasteur [Paris], Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos [Rio de Janeiro], Instituto Nacional de Infectologia Evandro Chagas [Rio de Janeiro] (INI), Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratório de Vigilância em Leishmanioses [Rio de Janeiro], Universidade Federal de São João del-Rei (UFSJ), This study was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) − Jovem Cientista do Nosso Estado. Fabiano Borges Figueiredo holds a grant from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for productivity in research., Institut Pasteur [Paris] (IP), Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Veterinary Parasitology
Veterinary Parasitology, Elsevier, 2016, 227, pp.8-14. ⟨10.1016/j.vetpar.2016.07.017⟩
Veterinary Parasitology, 2016, 227, pp.8-14. ⟨10.1016/j.vetpar.2016.07.017⟩
ISSN: 0304-4017
DOI: 10.1016/j.vetpar.2016.07.017⟩
Popis: International audience; In South America, visceral leishmaniasis is a zoonotic disease with severe evolution characteristics in humans, and dogs are its main reservoir. In this context, this study aimed to evaluate the clinical status of dogs from a Brazilian endemic area naturally, at Barra Mansa municipality, infected with Leishmania infantum, in conjunction with their histopathological profile and, in order to determine possible markers of susceptibility or resistance to the disease, parasitic DNA load, cytokine and iNOS mRNA expression profiles were investigated in lymph nodes. High levels of IFN-ɣ and IL-6 mRNA were detected. Both IFN-ɣ and IL-6 mRNA were associated with disorganization of the corticomedullary region. IFN-ɣ and TNF-α mRNA were associated with the absence of follicular hyperplasia. The regulatory pathway was remarkable with IL-10 mRNA detection and its significant association with the severity of the disease. Plasmacytosis and sinus histiocytosis were associated with high loads of parasitic DNA, but there was no significant association between the parasite DNA load and animal clinical alterations. Since high parasitic loads were found in animals with or without symptoms, clinical examination cannot be considered as a criterion for disease susceptibility assessment.
Databáze: OpenAIRE
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