Separation of three cyclic-nucleotide-phosphodiesterases from bovine aorta
| Autor: | Claire Lugnier, B. Ilien, Jean-Claude Stoclet, A. Stierlé, Y. Landry |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Activator (genetics)
Chemistry Kinetics Biophysics Cell Biology Cyclic nucleotide phosphodiesterases Biochemistry Calcium dependent digestive system diseases Substrate Specificity Isoenzymes Hydrolysis Media layer 3' 5'-Cyclic-AMP Phosphodiesterases 3' 5'-Cyclic-GMP Phosphodiesterases Substrate specificity Animals Cattle Molecular Biology Aorta Bovine aorta Cyclic IMP |
| Zdroj: | Biochemical and biophysical research communications. 83(2) |
| ISSN: | 0006-291X |
| Popis: | Three distinct forms of cyclic-nucleotide-phosphodiesterases were separated and purified from the media layer of bovine aorta using two successive DEAE-cellulose column chromatographies. Form A hydrolyzed both cAMP and cGMP with similar Km and Vmax and was sensitive to the calcium dependent protein activator; its activity on both substrates was inhibited by cIMP. Form B hydrolyzed specifically cGMP, was insensitive to the activator but was slightly stimulated in a proteinaceous medium; it was inhibited by cIMP. Form C was specific for cAMP and was insensitive to the activator and to a proteinaceous medium; it was poorly inhibited by cIMP. |
| Databáze: | OpenAIRE |
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