A Long-Lasting Calcium-Activated Nonselective Cationic Current Is Generated by Synaptic Stimulation or Exogenous Activation of Group I Metabotropic Glutamate Receptors in CA1 Pyramidal Neurons
| Autor: | Yehezkel Ben-Ari, Valérie Crépel, Patrice Congar, Xavier Leinekugel |
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| Přispěvatelé: | Epilepsie et Ischemie Cerebrale, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM), Tyzio, Roman, Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 1997 |
| Předmět: |
Male
Patch-Clamp Techniques Time Factors [SDV]Life Sciences [q-bio] Receptors Metabotropic Glutamate Synaptic Transmission 0302 clinical medicine MESH: Animals ComputingMilieux_MISCELLANEOUS 0303 health sciences Metabotropic glutamate receptor 5 Chemistry GABAA receptor Pyramidal Cells General Neuroscience Metabotropic glutamate receptor 6 Glutamate receptor food and beverages Articles MESH: Neuroprotective Agents Neuroprotective Agents MESH: Calcium Metabotropic glutamate receptor 1 MESH: Rats [SDV.BC]Life Sciences [q-bio]/Cellular Biology 03 medical and health sciences Cations MESH: Patch-Clamp Techniques MESH: Synaptic Transmission Animals Cycloleucine Rats Wistar MESH: Cycloleucine MESH: Receptors Metabotropic Glutamate MESH: Cations Reversal potential [SDV.BC] Life Sciences [q-bio]/Cellular Biology 030304 developmental biology Ion Transport fungi MESH: Time Factors MESH: Rats Wistar MESH: Pyramidal Cells MESH: Male Rats MESH: Ion Transport Metabotropic glutamate receptor Calcium Tetanic stimulation Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neuroscience Journal of Neuroscience, Society for Neuroscience, 1997, 17 (14), pp.5366-79 Journal of Neuroscience, Society for Neuroscience, 1997, 17 (14), pp.5366-5379. ⟨10.1523/JNEUROSCI.17-14-05366.1997⟩ ResearcherID |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.17-14-05366.1997 |
| Popis: | We have shown previously that a selective metabotropic glutamate receptor (mGluR) agonist, 1S,3R-1-aminocyclo-pentane-1,3-dicarboxylate (1S,3R-ACPD), evokes an inward current in CA1 pyramidal neurons of rat hippocampal slices in the presence of K+channel blockers (Crépel et al., 1994). This current has been characterized as a Ca2+-activated nonselective cationic (CAN) current. Using whole-cell patch-clamp recordings and intracellular dialysis, we now have identified the mGluR subtype and the mechanisms underlying the CAN current (ICAN) and report for the first time the presence of a synapticICANin the mammalian CNS. First, we have shown pharmacologically that activation ofICANby 1S,3R-ACPD involves the group I mGluRs (and not the groups II and III) and a G-protein-dependent process. We also report thatICANis modulated by the divalent cations (Mg2+, Cd2+, and Zn2+). Second, we have isolated a slow synaptic inward current evoked by a high-frequency stimulation in the presence of K+channel blockers, ionotropic glutamate, and GABAAreceptor antagonists. This current shows similar properties to the exogenously evokedICAN: its reversal potential is close to the reversal potential of the 1S,3R-ACPD-evokedICAN, and it is G-protein- and Ca2+-dependent. Because the amplitude and duration ofICANincreased in the presence of a glutamate uptake blocker, we suggest that this synaptic current is generated via the activation of mGluRs. We propose that the synapticICAN, activated by a brief tetanic stimulation and leading to a long-lasting inward current, may be involved in neuronal plasticity and synchronized network-driven oscillations. |
| Databáze: | OpenAIRE |
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