Characterization of an uncharacterized aldo-keto reductase gene from peach and its role in abiotic stress tolerance
| Autor: | Koki Kanahama, Shino Yaguchi, Hiroki Ikeda, Yoshinori Kanayama, Ayano Hojo, Ryosuke Mizutani, Manabu Nishiyama |
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| Rok vydání: | 2014 |
| Předmět: |
L-Iditol 2-Dehydrogenase
Molecular Sequence Data Aldo-Keto Reductases Arabidopsis Gene Expression Plant Science Horticulture Reductase Biochemistry Gene Expression Regulation Enzymologic chemistry.chemical_compound Aldehyde Reductase Gene Expression Regulation Plant Genes Reporter Stress Physiological Arabidopsis thaliana Amino Acid Sequence Molecular Biology Gene Abscisic acid Phylogeny Plant Proteins chemistry.chemical_classification Aldo-keto reductase biology Abiotic stress Salt Tolerance General Medicine Plants Genetically Modified biology.organism_classification Amino acid chemistry Organ Specificity Fruit Prunus Sequence Alignment |
| Zdroj: | Phytochemistry. 104:30-36 |
| ISSN: | 0031-9422 |
| Popis: | The aldo-keto reductase (AKR) superfamily is a large enzyme group of NADP-dependent oxidoreductases with numerous roles in metabolism, but many members in this superfamily remain uncharacterized. Here, PpAKR1, which was cloned from the rosaceous peach tree (Prunus persica), was investigated as a member of the superfamily. While PpAKR1 had amino acids that are important in AKRs and which belonged to the AKR4 group, PpAKR1 did not seem to belong to any of the AKR4 subgroups. PpAKR1 mRNA abundance increased with abscisic acid, oxidative stress, and cold and salt stress treatments in peach. NADP-dependent polyol dehydrogenase activity was increased in Arabidopsis thaliana transformed with PpAKR1. Salt tolerance increased in Arabidopsis transformed with PpAKR1. PpAKR1, which was a previously uncharacterized member of the AKR superfamily, could be involved in the abiotic stress tolerance. |
| Databáze: | OpenAIRE |
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