A fluorescence aptasensor based on two-dimensional sheet metal-organic frameworks for monitoring adenosine triphosphate
| Autor: | Ke Wang, Zhi-Qi Zhang, Nan Li, Jing Zhang, Fuquan Dang, Xiaoman Hai |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Cytidine triphosphate
Stereochemistry Aptamer Biosensing Techniques 02 engineering and technology Guanosine triphosphate 010402 general chemistry 01 natural sciences Biochemistry Fluorescence Photoinduced electron transfer Analytical Chemistry chemistry.chemical_compound Adenosine Triphosphate Spectroscopy Fourier Transform Infrared Environmental Chemistry Metal-Organic Frameworks Spectroscopy Uridine triphosphate Aptamers Nucleotide 021001 nanoscience & nanotechnology 0104 chemical sciences Fluorescein amidite chemistry Biophysics 0210 nano-technology Adenosine triphosphate |
| Zdroj: | Analytica Chimica Acta. 998:60-66 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2017.10.028 |
| Popis: | In the present study, a facile fluorescence aptasensor based on two-dimensional sheet metal-organic frameworks of N,N-bis(2-hydroxyethyl)dithiooxamidato copper(II) (H2dtoaCu) was developed for the sensitive detection of adenosine triphosphate (ATP). The sensing mechanism was based on the noncovalent interaction between FAM-labeled (fluorescein amidite) ATP aptamers and H2dtoaCu. In the absence of ATP, the FAM-labeled aptamer readily adsorbs onto H2dtoaCu, mainly via π-π stacking and hydrogen bond interactions between the nucleotide bases and the H2dtoaCu surface, leading to the reduction of fluorescence intensity of the FAM by photoinduced electron transfer (PET). In the presence of ATP, the FAM-labeled aptamer specifically forms ATP-binding aptamer complexes which exhibit only weak adsorption on the H2dtoaCu surface. Thus, the fluorescence of the FAM-labeled ATP aptamer remained largely unchanged. The fluorescence aptasensor exhibited a good linear relationship between the fluorescence intensity and the logarithm concentration of ATP over a range of 25–400 nM, with a detection limit of 8.19 nM (3S/N). ATP analogs such as guanosine triphosphate, uridine triphosphate, and cytidine triphosphate have negligible effect on the aptasensor performance due to the high selectivity of the ATP aptamer to its target, showing promising potential in real sample analysis. |
| Databáze: | OpenAIRE |
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