Variability in minimal genomes: Analysis of tandem repeats in the microsporidia Encephalitozoon intestinalis
| Autor: | Soledad Fenoy, A. Magnet, Fernando Izquierdo, Carmen del Aguila, Ana Luz Galván, Nuno Henriques-Gil |
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| Rok vydání: | 2013 |
| Předmět: |
Genetic Markers
Microbiology (medical) Genotype Molecular Sequence Data Polymorphism Single Nucleotide Microbiology Fungal Proteins Tandem repeat parasitic diseases Genetics Animals Humans Genetic variability Internal transcribed spacer DNA Fungal Molecular Biology Encephalitozoon cuniculi Ecology Evolution Behavior and Systematics Base Sequence biology fungi Encephalitozoon Genetic Variation Sequence Analysis DNA biology.organism_classification Encephalitozoon intestinalis Infectious Diseases Tandem Repeat Sequences Genetic marker Microsporidia Encephalitozoonosis Genome Fungal |
| Zdroj: | Infection, Genetics and Evolution. 20:26-33 |
| ISSN: | 1567-1348 |
| DOI: | 10.1016/j.meegid.2013.07.024 |
| Popis: | Microsporidia are ubiquitous fungi with genomes that have undergone a strong reduction to the extreme cases of Encephalitozoon cuniculi and Encephalitozoon intestinalis. Genetic variability within species of the Encephalitozoon genus has been reported, with most of the studies based on the internal transcribed spacer (ITS) of the rDNA. However, in contrast to the picture of E. cuniculi and Encephalitozoon hellem, where different strains have been identified, no genetic variability has yet been observed in E. intestinalis. We have analysed tandem repeats included in putative coding sequences which could be used as polymorphic markers in E. intestinalis. Eight candidate loci (M2, M2A, M3, M5, M7, M7A, M8 and PTP1) were established and 9 E. intestinalis cultured strains from North America, South America and Europe were analysed. M2, M7 and PTP1 nucleotide sequences were identical among the different strains and the GenBank sequence. In contrast, we observed variants in 4 markers (M2A, M3, M7A and M8) which did not correspond to their respective reference sequences. The most noticeable finding was that with the M5 marker two genotypes were defined among the different strains studied, demonstrating genotypic variability of E. intestinalis. Although the diversity described is certainly not high, which can be explained by a lower chance of genetic variability in its minimal genome, we have demonstrated that polymorphisms actually exist in E. intestinalis. Epidemiological studies using this genetic marker should now be conducted to elucidate the genetic variability in E. intestinalis and improve our knowledge of the epidemiology of this microsporidia. |
| Databáze: | OpenAIRE |
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