DNA aptamer-based colorimetric detection platform for Salmonella Enteritidis
| Autor: | Füsun Eyidoğan, Ceren Bayraç, Hüseyin Avni Öktem |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial Salmonella Aptamer Salmonella enteritidis Biomedical Engineering Biophysics Biosensing Techniques 02 engineering and technology Computational biology Biology DNA Aptamers medicine.disease_cause 01 natural sciences Microbiology chemistry.chemical_compound Electrochemistry medicine Humans Detection limit business.industry SELEX Aptamer Technique 010401 analytical chemistry General Medicine Aptamers Nucleotide 021001 nanoscience & nanotechnology Food safety 0104 chemical sciences 3. Good health chemistry Colorimetry 0210 nano-technology business Systematic evolution of ligands by exponential enrichment DNA Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 98:22-28 |
| ISSN: | 0956-5663 |
| Popis: | Food safety is a major issue to protect public health and a key challenge is to find detection methods for identification of hazards in food. Food borne infections affects millions of people each year and among pathogens, Salmonella Enteritidis is most widely found bacteria causing food borne diseases. Therefore, simple, rapid, and specific detection methods are needed for food safety. In this study, we demonstrated the selection of DNA aptamers with high affinity and specificity against S. Enteritidis via Cell Systematic Evolution of Ligands by Exponential Enrichment (Cell-SELEX) and development of sandwich type aptamer-based colorimetric platforms for its detection. Two highly specific aptamers, crn-1 and crn-2, were developed through 12 rounds of selection with Kd of 0.971µM and 0.309µM, respectively. Both aptamers were used to construct sandwich type capillary detection platforms. With the detection limit of 103 CFU/mL, crn-1 and crn-2 based platforms detected target bacteria specifically based on color change. This platform is also suitable for detection of S. Enteritidis in complex food matrix. Thus, this is the first to demonstrate use of Salmonella aptamers for development of the colorimetric aptamer-based detection platform in its identification and detection with naked eye in point-of-care. |
| Databáze: | OpenAIRE |
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