Increased glutathione levels contribute to the beneficial effects of hydrogen sulfide and inducible nitric oxide inhibition in allergic lung inflammation
| Autor: | Soraia K.P. Costa, Felipe G. Ravagnani, Heloisa H.A. Ferreira, Simone A. Teixeira, Anibal E. Vercesi, Daiana Campos, Sonia A. Gurgueira, Marcelo Nicolas Muscará |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Neutrophils Immunology Glutathione reductase Nitric Oxide Synthase Type II Sodium hydrosulfide medicine.disease_cause Aconitase FARMACOLOGIA Nitric oxide Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Cell Movement Internal medicine medicine Immunology and Allergy Animals Hydrogen Sulfide Enzyme Inhibitors Lung Pharmacology Aconitate Hydratase Mice Inbred BALB C medicine.diagnostic_test Glutathione Pneumonia respiratory system Asthma respiratory tract diseases Oxidative Stress 030104 developmental biology Bronchoalveolar lavage Endocrinology chemistry Biochemistry Female 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Objective The interaction between nitric oxide (NO) and hydrogen sulfide (H 2 S) in the airways could have significant implications for the pathogenesis and therapeutic effects of both on lung diseases. In this study we investigated whether the beneficial effects of H 2 S on asthma could be comparable to that inhibition of inducible NO synthase (iNOS). Methods Female BALB/C mice sensitized with ovalbumin (OVA) received either the H 2 S donor sodium hydrosulfide (NaHS, 14 μmol/kg) or the iNOS inhibitor 1400 W (1 mg/kg), 30 min before each OVA challenge during six days. On the first, second and sixth days, the leucocyte infiltration in lung parenchyma and bronchoalveolar lavage was evaluated. The aconitase activity (a sensor of O 2 formation) and lipid peroxidation, as well as levels of reduced glutathione (GSH) and oxidized glutathione (GSSG) were determined in the lung tissues. Results OVA-challenge caused a significant and time-dependent increase in the eosinophil number in the airways, which was accompanied by a significant decrease of aconitase activity and GSH/GSSG ratio along with enhanced lipid peroxidation in the lungs. Treatment with NaHS or 1400 W significantly attenuated the airways eosinophilia that was paralleled by an increase in aconitase activity and decrease of lipid peroxidation. NaHS or 1400 W treatments also reversed the decreased GSH/GSSG ratio seen after OVA-challenge. Conclusions The present study shows for the first time that the increased GSH/GSSG ratio caused by either H 2 S supplementation or iNOS-inhibition is a potential mechanism protecting airways against oxidative stress and inflammatory lung diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|