Genetic polymorphism of the swine major histocompatibility complex (SLA) class�I genes, SLA-1, -2 and -3
| Autor: | Hidetoshi Inoko, Shin-nosuke Takeshima, Nobuyuki Fujimura, Yoko Aida, Takahiro Iwanaga, Asako Ando, Tatsuya Anzai, Rieko Goto, Atsuko Shigenari, Masaharu Sada, Takashi Gojobori, Yoshiyuki Suzuki, H. Kawata, Yoshihiko Katsuyama, Masao Ota |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Complementary Genotype Swine Molecular Sequence Data Immunology Genes MHC Class I Miniature swine Human leukocyte antigen Biology Major histocompatibility complex Conserved sequence Antigen Sequence Homology Nucleic Acid Genetics Animals Humans Amino Acid Sequence Cloning Molecular Gene Alleles Conserved Sequence Phylogeny DNA Primers Polymorphism Genetic Base Sequence Sequence Homology Amino Acid Phylogenetic tree Reverse Transcriptase Polymerase Chain Reaction fungi Exons biology.protein RNA |
| Zdroj: | Immunogenetics. 55:583-593 |
| ISSN: | 1432-1211 0093-7711 |
| DOI: | 10.1007/s00251-003-0619-0 |
| Popis: | In order to identify and characterize genetic polymorphism of the swine major histocompatibility complex ( Mhc: SLA) class I genes, RT-PCR products of the second and third exons of the three SLA classical class I genes, SLA-1, SLA-2 and SLA-3 were subjected to nucleotide determination. These analyses allowed the identification of four, eight and seven alleles at the SLA-1, SLA-2 and SLA-3 loci, respectively, from three different breeds of miniature swine and one mixed breed. Among them, 12 alleles were novel. Construction of a phylogenetic tree using the nucleotide sequences of those 19 alleles indicated that the SLA-1 and -2 genes are more closely related to each other than to SLA-3. Selective forces operating at single amino acid sites of the SLA class I molecules were analyzed by the Adaptsite Package program. Ten positive selection sites were found at the putative antigen recognition sites (ARSs). Among the 14 positively selected sites observed in the human MHC ( HLA) classical class I molecules, eight corresponding positions in the SLA class I molecules were inferred as positively selected. On the other hand, four amino acids at the putative ARSs were identified as negatively selected in the SLA class I molecules. These results suggest that selective forces operating in the SLA class I molecules are almost similar to those of the HLA class I molecules, although several functional sites for antigen and cytotoxic T-lymphocyte recognition by the SLA class I molecules may be different from those of the HLA class I molecules. |
| Databáze: | OpenAIRE |
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