MOESM1 of Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region

Autor: Bendezu, Jorge, Villasis, Elizabeth, Ruiz, Sandra Morales, Garro, Katherine, Berónica Infante, Gutierrez-Loli, Renzo, Rodríguez, Pamela, Fernández-Díaz, Manolo, Dionicia Gamboa, Torres, Katherine
Rok vydání: 2019
DOI: 10.6084/m9.figshare.9894671
Popis: Additional file 1: Figure S1. The Codon–optimized pfmsp10 gene (79–1518 nu). Figure S2. Construction of the pFastBac-pfmsp10 vector with insertions of codon-optimized pfmsp10 gene (from pUC57 vector). Figure S3. Standard-curve of P. falciparum serum pool using either: a) rMSP10, b) PfMSP10-1 or b) PfMSP10-2 as antigen in ELISA assays. Figure S4. Amino acid sequence alignment between PfMSP10 and PvMSP10 proteins. Figure S5. rMSP10 cross-reaction against healthy donor sera. Figure S6. rMSP10 cross-reaction against other non-Plasmodium spp. infection sera and healthy donor sera. Figure S7. BLASTp results using PfMSP10 aminoacide sequence against to Leishmania (taxid:5658) or Leptospira (taxid:37387) protein data base. Table S1. List of antigenic peptides selected by Optimum Antigen using the amino acid sequence of PfMSP10 (GenBank:XP_966190.1).
Databáze: OpenAIRE