Immunization of Broiler Chickens against Clostridium perfringens -Induced Necrotic Enteritis
Autor: | Raveendra R. Kulkarni, Valeria R. Parreira, Shayan Sharif, John F. Prescott |
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Rok vydání: | 2007 |
Předmět: |
Male
Microbiology (medical) animal structures Clostridium perfringens Pyruvate Synthase Bacterial Toxins Clinical Biochemistry Immunology Virulence Biology medicine.disease_cause Veterinary Immunology Microbiology Enteritis Necrosis Immunity Fructose-Bisphosphate Aldolase Intestine Small medicine Animals Immunology and Allergy Cloning Molecular Poultry Diseases Toxin Calcium-Binding Proteins Antibody titer Glyceraldehyde-3-Phosphate Dehydrogenases medicine.disease Antibodies Bacterial Virology Recombinant Proteins Bacterial vaccine Type C Phospholipases Bacterial Vaccines Clostridium Infections biology.protein Antibody Chickens |
Zdroj: | Clinical and Vaccine Immunology. 14:1070-1077 |
ISSN: | 1556-679X 1556-6811 |
Popis: | Necrotic enteritis (NE) in broiler chickens is caused by Clostridium perfringens . Currently, no vaccine against NE is available and immunity to NE is not well characterized. Our previous studies showed that immunity to NE followed oral infection by virulent rather than avirulent C. perfringens strains and identified immunogenic secreted proteins apparently uniquely produced by virulent C. perfringens isolates. These proteins were alpha-toxin, glyceraldehyde-3-phosphate dehydrogenase, pyruvate:ferredoxin oxidoreductase (PFOR), fructose 1,6-biphosphate aldolase, and a hypothetical protein (HP). The current study investigated the role of each of these proteins in conferring protection to broiler chickens against oral infection challenges of different severities with virulent C. perfringens . The genes encoding these proteins were cloned and purified as histidine-tagged recombinant proteins from Escherichia coli and were used to immunize broiler chickens intramuscularly. Serum and intestinal antibody responses were assessed by enzyme-linked immunosorbent assay. All proteins significantly protected broiler chickens against a relatively mild challenge. In addition, immunization with alpha-toxin, HP, and PFOR also offered significant protection against a more severe challenge. When the birds were primed with alpha-toxoid and boosted with active toxin, birds immunized with alpha-toxin were provided with the greatest protection against a severe challenge. The serum and intestinal washings from protected birds had high antigen-specific antibody titers. Thus, we conclude that there are certain secreted proteins, in addition to alpha-toxin, that are involved in immunity to NE in broiler chickens. |
Databáze: | OpenAIRE |
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