Age-dependent regulation of ERF-VII transcription factor activity in Arabidopsis thaliana
Autor: | Lara Lombardi, Beatrice Giuntoli, Francesco Licausi, Vinay Shukla, Federica Maggiorelli, Federico M. Giorgi, Pierdomenico Perata |
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Přispěvatelé: | Giuntoli, Beatrice, Shukla, Vinay, Maggiorelli, Federica, Giorgi, Federico M., Lombardi, Lara, Perata, Pierdomenico, Licausi, Francesco |
Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
0301 basic medicine Transcription Factor Physiology Arabidopsis Repressor Plant Science Biology Genes Plant 01 natural sciences Transcriptome 03 medical and health sciences flooding seedling-to-adult plant development Gene Expression Regulation Plant Gene expression Transcriptional regulation oxidative stress signalling Promoter Regions Genetic hypoxia N-end rule pathway transcriptome Transcription factor Gene Sequence Deletion Genetics oxidative stre Base Sequence fungi food and beverages biology.organism_classification Cell biology Mitochondria Plant Leaves Metabolic pathway 030104 developmental biology Phenotype Arabidopsi Plant Leave 010606 plant biology & botany Transcription Factors |
Popis: | The Group VII Ethylene Responsive Factors (ERFs-VII) RAP2.2 and RAP2.12 have been mainly characterized with regard to their contribution as activators of fermentation in plants. However, transcriptional changes measured in conditions that stabilize these transcription factors exceed the mere activation of this biochemical pathway, implying additional roles performed by the ERF-VIIs in other processes. We evaluated gene expression in transgenic Arabidopsis lines expressing a stabilized form of RAP2.12, or hampered in ERF-VII activity, and identified genes affected by this transcriptional regulator and its homologs, including some involved in oxidative stress response, which are not universally induced under anaerobic conditions. The contribution of the ERF-VIIs in regulating this set of genes in response to chemically induced or submergence-stimulated mitochondria malfunctioning was found to depend on the plant developmental stage. A similar age-dependent mechanism also restrained ERF-VII activity upon the core-hypoxic genes, independently of the N-end rule pathway, which is accounted for the control of the anaerobic response. To conclude, this study shed new light on a dual role of ERF-VII proteins under submergence: as positive regulators of the hypoxic response and as repressors of oxidative-stress related genes, depending on the developmental stage at which plants are challenged by stress conditions. |
Databáze: | OpenAIRE |
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