Antigenic Structure of Outer Membrane Protein E of Moraxella catarrhalis and Construction and Characterization of Mutants
| Autor: | Aimee L. Brauer, Timothy F. Murphy, Norine Yuskiw, Thomas J. Hiltke |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Blood Bactericidal Activity
Antigenicity medicine.drug_class Immunology Monoclonal antibody complex mixtures Microbiology Epitope Moraxella catarrhalis Mice Antigen Moraxella (Branhamella) catarrhalis medicine Animals Antigens Bacterial Mice Inbred BALB C biology Antibodies Monoclonal Bacterial Infections bacterial infections and mycoses Flow Cytometry biology.organism_classification Infectious Diseases Mutation biology.protein bacteria Parasitology Antibody Bacterial outer membrane Bacterial Outer Membrane Proteins |
| Zdroj: | Infection and Immunity. 68:6250-6256 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.68.11.6250-6256.2000 |
| Popis: | Outer membrane protein E (OMP E) is a 50-kDa protein of Moraxella catarrhalis which possesses several characteristics indicating that the protein will be an effective vaccine antigen. To study the antigenic structure of OMP E, eight monoclonal antibodies were developed and characterized. Three of the antibodies recognized epitopes which are present on the bacterial surface. Fusion peptides corresponding to overlapping regions of OMP E were constructed, and immunoblot assays were performed to localize the areas of the molecule bound by the monoclonal antibodies. These studies identified a surface-exposed epitope in the region of amino acids 80 through 180. To further study the protein, two mutants which lack OMP E were constructed. In bactericidal assays, the mutants were more readily killed by normal human serum compared to the isogenic parent strains. These results indicate that OMP E is involved in the expression of serum resistance of M. catarrhalis . |
| Databáze: | OpenAIRE |
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