Homology-Dependent Maternal Inhibition of Developmental Excision of Internal Eliminated Sequences in Paramecium tetraurelia
| Autor: | Sandra Duharcourt, Eric Meyer, Anne-Marie Keller |
|---|---|
| Přispěvatelé: | Régulation de l'expression génétique (REG), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut National des Langues et Civilisations Orientales (Inalco) |
| Rok vydání: | 1998 |
| Předmět: |
Sequence analysis
[SDV]Life Sciences [q-bio] Genes Protozoan Gene Dosage Cell Line Conserved sequence 03 medical and health sciences Transformation Genetic Nuclear dimorphism Animals Direct repeat [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Paramecium Molecular Biology Gene ComputingMilieux_MISCELLANEOUS Sequence Deletion 030304 developmental biology Genetics 0303 health sciences biology Macronucleus 030302 biochemistry & molecular biology DNA Sequence Analysis DNA Cell Biology DNA Protozoan biology.organism_classification DNA Dynamics and Chromosome Structure Bacteriophage lambda Germ Cells Oligodeoxyribonucleotides Paramecium tetraurelia Programmed DNA elimination Sequence Alignment |
| Zdroj: | Molecular and Cellular Biology Molecular and Cellular Biology, American Society for Microbiology, 1998, 18 (12), pp.7075-7085. ⟨10.1128/MCB.18.12.7075⟩ |
| ISSN: | 1098-5549 0270-7306 |
| Popis: | Thousands of single-copy internal eliminated sequences (IESs) are excised from the germ line genome of ciliates during development of the polygenomic somatic macronucleus, following sexual events. Paramecium IESs are short, noncoding elements that frequently interrupt coding sequences. No absolutely conserved sequence element, other than flanking 5'-TA-3' direct repeats, has been identified among sequenced IESs; the mechanisms of their specific recognition and precise elimination are unknown. Previous work has revealed the existence of an epigenetic control of excision. It was shown that the presence of one IES in the vegetative macronucleus results in a specific inhibition of the excision of the same element during the development of a new macronucleus, in the following sexual generation. We have assessed the generality and sequence specificity of this transnuclear maternal control by studying the effects of macronuclear transformation with 13 different IESs. We show that at least five of them can be maintained in the new macronuclear genome; sequence specificity is complete both between genes and between different IESs in the same gene. In all cases, the degree of excision inhibition correlates with the copy number of the maternal IES, but each IES shows a characteristic inhibition efficiency. Short internal IES-like segments were found to be excised from two of the IESs when excision between normal boundaries was inhibited. Available data suggest that the sequence specificity of these maternal effects is mediated by pairing interactions between homologous nucleic acids. |
| Databáze: | OpenAIRE |
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