Long noncoding RNA Sox2ot and transcription factor YY1 co-regulate the differentiation of cortical neural progenitors by repressing Sox2
| Autor: | Jennifer L. Knauss, Tao Sun, Mary E. Donohoe, Hugo Pinto, Yuelin Shi, Nan Miao, Seung-Nam Kim, Tao Wu, Yanzhen Nie |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Neurogenesis Cellular differentiation Immunology Biology Article SOX2OT Mice 03 medical and health sciences Cellular and Molecular Neuroscience Neural Stem Cells SOX2 Transcriptional regulation Animals Cell Self Renewal RNA Small Interfering lcsh:QH573-671 YY1 Transcription Factor Cell Nucleus Cerebral Cortex lcsh:Cytology SOXB1 Transcription Factors Cell Differentiation Cell Biology Long non-coding RNA Neural stem cell Cell biology 030104 developmental biology embryonic structures CpG Islands RNA Interference RNA Long Noncoding Stem cell Protein Binding |
| Zdroj: | Cell Death and Disease, Vol 9, Iss 8, Pp 1-13 (2018) Cell Death & Disease |
| ISSN: | 2041-4889 |
| DOI: | 10.1038/s41419-018-0840-2 |
| Popis: | Long noncoding RNAs (lncRNAs) are emerging as key regulators of crucial cellular processes. However, the molecular mechanisms of many lncRNA functions remain uncharacterized. Sox2ot is an evolutionarily conserved lncRNA that transcriptionally overlaps the pluripotency gene Sox2, which maintains the stemness of embryonic stem cells and tissue-specific stem cells. Here, we show that Sox2ot is expressed in the developing mouse cerebral cortex, where it represses neural progenitor (NP) proliferation and promotes neuronal differentiation. Sox2ot negatively regulates self-renewal of neural stem cells, and is predominately expressed in the nucleus and inhibits Sox2 levels. Sox2ot forms a physical interaction with a multifunctional transcriptional regulator YY1, which binds several CpG islands in the Sox2 locus in a Sox2ot-dependent manner. Similar to Sox2ot, YY1 represses NP expansion in vivo. These results demonstrate a regulatory role of Sox2ot in promoting cortical neurogenesis, possibly by repressing Sox2 expression in NPs, through interacting with YY1. |
| Databáze: | OpenAIRE |
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