Using Prenatal Blood Samples to Evaluate COVID-19 Rapid Serologic Tests Specificity

Autor: Emily W. Harville, A. Corrales, Maria Luisa Cafferata, Luz Gibbons, Xu Xiong, Dahlene N. Fusco, Fausto Muñoz-Lara, Pierre Buekens, Arnaud Drouin, Hans Desale, Jackeline Alger, Ivette Lorenzana, Wendy Lopez, Elsa Palou, Candela Stella, Lysien I. Zambrano, Manuel Sierra, Alvaro Ciganda, Tito Alvarado, Eduardo Retes, Jorge García
Rok vydání: 2020
Předmět:
Adult
medicine.medical_specialty
Adolescent
From the Field
Epidemiology
Population
Pneumonia
Viral
Serologic tests
Prenatal care
Sensitivity and Specificity
Serology
COVID-19 diagnostic testing
Cohort Studies
03 medical and health sciences
Betacoronavirus
Young Adult
0302 clinical medicine
COVID-19 Testing
Predictive Value of Tests
Pregnancy
Internal medicine
Obstetrics and Gynaecology
medicine
Humans
Pediatrics
Perinatology
and Child Health
030212 general & internal medicine
education
Pandemics
education.field_of_study
030219 obstetrics & reproductive medicine
business.industry
Clinical Laboratory Techniques
SARS-CoV-2
Public Health
Environmental and Occupational Health
Obstetrics and Gynecology
COVID-19
Prenatal Care
medicine.disease
Confidence interval
Predictive value of tests
Pediatrics
Perinatology and Child Health
Cohort
Female
business
Coronavirus Infections
Cohort study
Zdroj: Maternal and Child Health Journal
ISSN: 1573-6628
Popis: Introduction Background cross-reactivity with other coronaviruses may reduce the specificity of COVID-19 rapid serologic tests. The vast majority of women attend prenatal care, which is a unique source of population-based blood samples appropriate for validation studies. We used stored 2018 serum samples from an existing pregnancy cohort study to evaluate the specificity of COVID-19 serologic rapid diagnostic tests. Methods We randomly selected 120 stored serum samples from pregnant women enrolled in a cohort in 2018 in Tegucigalpa, Honduras, at least 1 year before the COVID-19 pandemic. We used stored serum to evaluate four lateral flow rapid diagnostic tests, following manufacturers’ instructions. Pictures were taken for all tests and read by two blinded trained evaluators. Results We evaluated 120, 80, 90, and 90 samples, respectively. Specificity for both IgM and IgG was 100% for the first two tests (95% confidence intervals [CI] 97.0–100 and 95.5–100, respectively). The third test had a specificity of 98.9% (95% CI 94.0–100) for IgM and 94.4% (95% CI 87.5–98.2) for IgG. The fourth test had a specificity of 88.9% (95% CI 80.5–94.5) for IgM and 100% (95% CI 96.0–100) for IgG. Discussion COVID-19 serologic rapid tests are of variable specificity. Blood specimens from sentinel prenatal clinics provide an opportunity to validate serologic tests with population-based samples.
Databáze: OpenAIRE
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