Identification of subregions of Bordetella pertussis filamentous hemagglutinin that stimulate human T-cell responses
| Autor: | Mario Domenighini, M Bugnoli, M T De Magistris, A Di Tommaso, A Tagliabue, Rino Rappuoli |
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| Rok vydání: | 1991 |
| Předmět: |
Cellular immunity
Bordetella pertussis T-Lymphocytes T cell Molecular Sequence Data Immunology Filamentous haemagglutinin adhesin Cross Reactions Biology Lymphocyte Activation Microbiology Epitopes medicine Humans Amino Acid Sequence Virulence Factors Bordetella Adhesins Bacterial Peptide sequence Hemagglutinin biology.organism_classification Peptide Fragments Recombinant Proteins Bordetella Bacterial adhesin Hemagglutinins Infectious Diseases medicine.anatomical_structure Parasitology Oligopeptides Research Article |
| Zdroj: | Infection and Immunity. 59:3313-3315 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Filamentous hemagglutinin (FHA), a 220-kDa protein that mediates the adhesion of Bordetella pertussis to eukaryotic cells, is a component of acellular vaccines against whooping cough. To identify the subregions of FHA that are immunogenic for T cells, 16 human T-cell clones were raised against purified FHA and tested for the recognition of recombinant and proteolytic fragments. The clones were found to map either in the carboxy-terminal or the amino-terminal part of the FHA molecule, but none of them recognized the central region, which contains a sequence that is homologous to that of the eukaryotic protein fibronectin. These data suggest that subregions of FHA that do not contain sequences that are potentially cross-reactive with self proteins may be sufficient to induce an immune response against the whole protein. |
| Databáze: | OpenAIRE |
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