The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons
| Autor: | Hannah Monyer, Wytse J. Wadman, Dragos Inta, Tania Vitalis, Johannes A. van Hooft, Pascal Chameau |
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| Přispěvatelé: | Cellular and Computational Neuroscience (SILS, FNWI) |
| Rok vydání: | 2009 |
| Předmět: |
Cell Adhesion Molecules
Neuronal Cellular differentiation Nerve Tissue Proteins Biology Cell Line Tissue Culture Techniques Mice Animals Humans Reelin Receptor 5-HT receptor Mice Knockout Extracellular Matrix Proteins Multidisciplinary Pyramidal Cells Serine Endopeptidases Cell Differentiation Dendrites Biological Sciences DAB1 Cortex (botany) Cell biology Mice Inbred C57BL Reelin Protein Animals Newborn nervous system Knockout mouse Immunology biology.protein Receptors Serotonin 5-HT3 Signal transduction |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, 106(17), 7227-7232. National Academy of Sciences |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0810764106 |
| Popis: | Cajal-Retzius cells, located in layer I of the cortex, synthesize and secrete the glycoprotein reelin, which plays a pivotal role in neuronal migration during embryonic development. Cajal-Retzius cells persist after birth, but their postnatal role is unknown. Here we show that Cajal-Retzius cells receive a major excitatory synaptic input via serotonin 5-HT 3 receptors. Blocking this input using pharmacological tools or neutralization of reelin signaling results in hypercomplexity of apical, but not basal, dendrites of cortical layer II/III pyramidal neurons. A similar hypercomplexity is observed in the cortex of the 5-HT 3A receptor knockout mouse. The increased dendritic complexity can be rescued by application of recombinant full-length reelin or its N-terminal fragment, but not by the central fragment of reelin, and involves a signal transduction pathway independent of the activation of the canonical reelin receptors. Taken together, our results reveal a novel role of serotonin, Cajal-Retzius cells, and reelin in the postnatal maturation of the cortex. |
| Databáze: | OpenAIRE |
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