Blood Microbiome Quantity and the Hyperdynamic Circulation in Decompensated Cirrhotic Patients
| Autor: | Martina Buck, Beacher Schneider, Mario Chojkier, Daniela Traykova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Liver Cirrhosis Male Cardiac output Cirrhosis Physiology Hemodynamics lcsh:Medicine Artificial Gene Amplification and Extension Pathology and Laboratory Medicine Gastroenterology Biochemistry Polymerase Chain Reaction chemistry.chemical_compound Liver Function Tests Medicine and Health Sciences Medicine lcsh:Science Immune Response Multidisciplinary Liver Diseases Microbiota Neurochemistry Hematology Genomics Middle Aged 3. Good health Body Fluids medicine.anatomical_structure Blood Medical Microbiology Hyperdynamic circulation Cytokines medicine.symptom Anatomy Neurochemicals Research Article medicine.medical_specialty Immunology Inflammation Microbial Genomics Gastroenterology and Hepatology Nitric Oxide Research and Analysis Methods Microbiology Nitric oxide 03 medical and health sciences Signs and Symptoms Diagnostic Medicine Internal medicine Sepsis NLR Family Pyrin Domain-Containing 3 Protein Genetics Humans Intensive care medicine Molecular Biology Techniques Molecular Biology Aged Bacteria business.industry Macrophages lcsh:R Case-control study Organisms Biology and Life Sciences medicine.disease Gastrointestinal Microbiome 030104 developmental biology chemistry Gene Expression Regulation Case-Control Studies Vascular resistance Metagenome lcsh:Q Microbiome Metagenomics business Biomarkers Neuroscience |
| Zdroj: | PLoS ONE, Vol 12, Iss 2, p e0169310 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background Recently, a complex microbiome was comprehensibly characterized in the serum and ascitic fluid of cirrhotic patients. In the current study, we investigated for the first time the induction of inflammatory pathways and Nitric Oxide, as well as the systemic hemodynamics in conjunction with the blood microbiome in a Child-Pugh class B cirrhotic cohort. Methods and Findings We used the Intestinal Infections Microbial DNA qPCR Array to screen for 53 bacterial DNA from the gut in the blood. Assays were designed using the 16S rRNA gene as a target, and PCR amplification primers (based on the Human Microbiome Project) and hydrolysis-probe detection. Eighteen systemic hemodynamic parameters were measured non-invasively by impedance cardiography using the BioZ ICG monitor. The inflammatory response was assessed by measuring blood cytokines, Nitric Oxide RNA arrays, and Nitric Oxide. In the blood of this cirrhotic cohort, we detected 19 of 53 bacterial species tested. The number of bacterial species was markedly increased in the blood of cirrhotic patients compared to control individuals (0.2+/-0.4 vs 3.1+/-2.3; 95% CI: 1.3 to 4.9; P = 0.0030). The total bacterial DNA was also increased in the blood of cirrhotic subjects compared to control subjects (0.2+/- 1.1 vs 41.8+/-132.1; 95% CI: 6.0 to 77.2; P = 0.0022). In the cirrhotic cohort, the Cardiac Output increased by 37% and the Systemic Vascular Resistance decreased by 40% (P< 0.00001 for both compared to control subjects). Systemic Vascular Resistance was inversely correlated to blood bacterial DNA quantity (- 0.621; 95% CI -0.843 to -0.218; P = 0.0060), blood bacterial species number (- 0.593; 95% CI -0.83 to -0.175; P = 0.0095; logistic regression: Chi Square = 5.8877; P = 0.0152), and serum Nitric Oxide (- 0.705; 95% CI -0.881 to -0.355; P = 0.0011). Many members of the Nitric Oxide signaling pathway gene family were increased in cirrhotic subjects. Conclusions Our study identified blood bacterial DNA in ~ 90% of the cirrhotic patients without clinical evidences of infection, and suggests that the quantity of bacterial DNA in blood may stimulate signaling pathways, including Nitric Oxide, that could decrease systemic vascular resistance and increase cardiac output. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|