BCR-ABL activity measured by 50% inhibitory concentration for imatinib, p-CrkL/CrkL ratio or p-CrkL ratio in CD34+ cells of patients with chronic myeloid leukemia does not predict treatment response
| Autor: | Dana Dvorakova, Filip Rázga, Irena Koutná, Tomáš Jurček, Pavel Šimara, Jiri Mayer, Zdenek Racil, Michaela Potesilova, Stanislav Stejskal, Martina Peterková |
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| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
CD34 Fusion Proteins bcr-abl Antigens CD34 Antineoplastic Agents Models Biological Piperazines Flow cytometry 03 medical and health sciences Inhibitory Concentration 50 0302 clinical medicine hemic and lymphatic diseases Leukemia Myelogenous Chronic BCR-ABL Positive medicine Humans Kinase activity Adaptor Proteins Signal Transducing medicine.diagnostic_test business.industry Gene Expression Regulation Leukemic Myeloid leukemia Nuclear Proteins Imatinib Hematology Flow Cytometry In vitro 3. Good health CRKL Pyrimidines Treatment Outcome Oncology 030220 oncology & carcinogenesis Benzamides Cancer research Imatinib Mesylate Phosphorylation business 030215 immunology medicine.drug Signal Transduction |
| Zdroj: | Leukemialymphoma. 53(8) |
| ISSN: | 1029-2403 |
| Popis: | In this study, we assessed BCR-ABL kinase activity by measuring the protein levels of CrkL and its phosphorylated form (p-CrkL) in order to predict the clinical outcome of newly diagnosed chronic myeloid leukemia patients. CD34+ cells from these patients were collected before the start of imatinib therapy and treated in vitro with imatinib. The reduction of p-CrkL and CrkL protein levels was then measured by flow cytometry. The data were processed using three independent approaches: an assessment of a) IC50imatinib, b) p-CrkL/CrkL ratio, and c) p-CrkL ratio. The results were subsequently correlated with the clinical response of patients after 3 and 6 months of imatinib therapy. None of the three p-CrkL parameters measured in CD34+ cells was found to be predictive of clinical outcome. |
| Databáze: | OpenAIRE |
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