A Genetic Map for the Only Self-Fertilizing Vertebrate
| Autor: | Yoshitaka Sakakura, Yasufumi Yuasa, Angel Amores, Kouichi Kawamura, Takamasa Suzuki, Koushirou Suga, Katsuyasu Kamimura, Yosuke Sugita, Catherine Wilson, John H. Postlethwait, Yoshinobu Uno, Yoichi Matsuda, Akira Kanamori |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetic Linkage Centromere Self-Fertilization QH426-470 Biology Synteny Evolution Molecular 03 medical and health sciences Chromosome 16 hermaphrodite Chromosome 18 Chromosome 19 Genetics Animals 14. Life underwater Molecular Biology Genetics (clinical) Chromosome 12 Genetic Association Studies Phylogeny phylogeny by RAD-seq Recombination Genetic teleost Genome Chromosome Mapping Genomics Chromosome 17 (human) Genetics of Sex 030104 developmental biology Chromosome 4 Chromosome 3 Vertebrates centromeres and recombination Chromosome 21 conserved chromosomes |
| Zdroj: | G3: Genes|Genomes|Genetics G3: Genes, Genomes, Genetics, Vol 6, Iss 4, Pp 1095-1106 (2016) |
| ISSN: | 2160-1836 |
| Popis: | The mangrove killifish Kryptolebias marmoratus, and its close relative Kryptolebias hermaphroditus, are the only vertebrate species known to reproduce by self-fertilization due to functional ovotestis development. To improve our understanding of their genomes, we constructed a genetic map. First, a single F1 fish was made by artificial fertilization between K. marmoratus and K. hermaphroditus strains. F2 progeny were then obtained by self-fertilization of the F1 fish. We used RAD-seq to query genomic DNAs from the two parental strains, the F1 individual and 49 F2 progeny. Results identified 9904 polymorphic RAD-tags (DNA markers) that mapped to 24 linkage groups, corresponding to the haploid chromosome number of these species. The total length of the map was 1248 cM, indicating that about one recombination occurred for each of the 24 homologous chromosome pairs in each meiosis. Markers were not evenly distributed along the chromosomes: in all chromosomes, many markers (> 8% of the total markers for each chromosome) mapped to chromosome tips. Centromeres suppress recombination, and this uneven distribution is probably due to the species’ acrocentric chromosomes. Mapped marker sequences were compared to genomic sequences of medaka and platyfish, the next most closely related species with sequenced genomes that are anchored to genetic maps. Results showed that each mangrove killifish chromosome corresponds to a single chromosome of both platyfish and medaka, suggesting strong conservation of chromosomes over 100 million years of evolution. Our genetic map provides a framework for the K. marmoratus/K. hermaphroditus genome sequence and an important resource for understanding the biology of hermaphroditism. |
| Databáze: | OpenAIRE |
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