Upregulation of sphingosine kinase�1 contributes to�ventilator-associated lung injury in a two-hit model
| Autor: | Zhou Lv, Xiaoyan Zhu, Yan Wang, Wen‑Wen Dong, Dun‑Feng Xu, Yu‑Jian Liu, Ting‑Ting Gao, Lai Jiang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Ventilator-associated lung injury sphingosine kinase 1 Ventilator-Induced Lung Injury medicine.medical_treatment Intraperitoneal injection Lung injury Pharmacology ras homolog family member a Capillary Permeability Mice Myosin-Light-Chain Phosphatase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Sphingosine Intensive care Genetics medicine Animals Humans Sphingosine-1-phosphate Phosphorylation Evans Blue rho-Associated Kinases biology medicine.diagnostic_test Chemistry mechanical stretch Endothelial Cells Articles General Medicine medicine.disease Disease Models Animal Phosphotransferases (Alcohol Group Acceptor) 030104 developmental biology Bronchoalveolar lavage Gene Expression Regulation acute lung injury Sphingosine kinase 1 030220 oncology & carcinogenesis biology.protein endothelial permeability Lysophospholipids Bronchoalveolar Lavage Fluid Signal Transduction |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| Popis: | Ventilator-associated lung injury (VALI) remains a significant medical problem in intensive care units. The present study aimed to investigate the role of sphingosine kinase 1 (SPHK1) in VALI using a two-hit model and explore the potential underlying molecular mechanism. Mice were divided into five groups: i) Non-ventilated group; ii) non-ventilated + lipopolysaccharide (LPS) group; iii) ventilated group; iv) ventilated + LPS group; and v) ventilated + LPS + SPHK1 inhibitor group. Mice were administered LPS (1 mg/kg) via an intraperitoneal injection. After 12 h, the mice were anesthetized and connected to a ventilator (10 ml/kg at 150 breaths/min) for 4 h. SPHK1 inhibitor (50 mg/kg) was injected intraperitoneally 1 h prior to ventilation. Mouse lung vascular endothelial cells were treated with LPS and SPHK1 inhibitor, and then subjected to cyclic stretch for 4 h. The present results suggested that the expression of SPHK1 and sphingosine 1 phosphate was upregulated in the two-hit model of VALI; SPHK1 inhibitor could attenuate VALI in the two-hit model as observed by hematoxylin and eosin staining, and affected the cell count and the protein content levels in the bronchoalveolar lavage fluid. In addition, treatment with SPHK1 inhibitor reduced the wet-to-dry ratio of the lungs and suppressed Evans blue dye leakage into the lung tissue. Furthermore, SPHK1 inhibitor exhibited protective effects on the two-hit model of VALI by inhibiting the Ras homolog family member a-mediated phosphorylation of myosin phosphatase target subunit 1 (MYPT-1) and endothelial hyperpermeability. Additionally, mice were divided into five additional groups: i) Non-ventilated group; ii) non-ventilated + LPS group; iii) ventilated group; iv) ventilated + LPS group; and v) ventilated + LPS + Rho-associated coiled-coil forming protein kinase (ROCK)1 inhibitor group. ROCK1 inhibitor (10 mg/kg) was injected intraperitoneally 1 h prior to ventilation. The present results suggested that ROCK1 inhibitor could attenuate mechanical stretch-induced lung endothelial injury and the phosphorylation of MYPT-1 in vivo and in vitro. Collectively, the present findings indicated that upregulation of SPHK1 may contribute to VALI in a two-hit model. |
| Databáze: | OpenAIRE |
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