Rapid and ultrasensitive method for determination of aflatoxin M1 in milk
| Autor: | Shi Longshun, Zhou Yan, Jun Fan, Yi Zhang, Bin Zhou, Jue Zhang, Li Wenxin, Xueyu Yuan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
Aflatoxin milk Chromatography Specific test aflatoxin m1 Chemistry 010401 analytical chemistry Immunology 04 agricultural and veterinary sciences Time resolved fluorescence immunoassay 040401 food science 01 natural sciences time-resolved fluorescence immunoassay lcsh:S1-972 0104 chemical sciences 0404 agricultural biotechnology Fluorescent microspheres fluorescent microsphere lcsh:Agriculture (General) lcsh:RC581-607 Agronomy and Crop Science Food Science poct |
| Zdroj: | Food and Agricultural Immunology, Vol 31, Iss 1, Pp 849-858 (2020) |
| ISSN: | 1465-3443 0954-0105 |
| Popis: | Aflatoxin M1 (AFM1) in milk is a problem of great concern. Current methods of detection require large instruments and need specific test sites. Therefore, it is necessary to establish a fast, convenient, and accurate detection method for AFM1. We established a system based on fluorescent microspheres containing a Eu3+ chelate named AFM1-POCT. These components comprised the AFM1-POCT kits. After refrigeration at 4°C for 12 months, the intra and inter assay coefficients of variability (CVs) for the kits were 4% and 5%, respectively. AFM1-POCT compared well with ultra-high-performance liquid chromatography (UHPLC) determination in the range 0.0121–2 μg/kg (paired samples test, P > 0.05). Aflatoxin B1 and G1 do not react with aflatoxin M1. By using the AFM1-POCT method, the detection time is shortened to 5 min, the accuracy is comparable to that of UHPLC, and convenience and range of application are improved. |
| Databáze: | OpenAIRE |
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