The Application of Molecular Diagnostics to Stained Cytology Smears
| Autor: | Laleh Hakima, Evan Pieri, Mark J. Suhrland, Antonio Cajigas, Esther Adler, Andrew W. Seymour, Eli Grunblatt, Samer N. Khader, Maja H. Oktay, Sumanta Goswami |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Proto-Oncogene Proteins B-raf 0301 basic medicine Thyroid nodules Pathology medicine.medical_specialty Oncogene Proteins Fusion Cytodiagnosis DNA Mutational Analysis Real-Time Polymerase Chain Reaction Bioinformatics Translocation Genetic Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Neoplasms Cytology Biomarkers Tumor medicine Humans Mutation detection Aged Aged 80 and over Lung business.industry Thyroid Middle Aged Molecular diagnostics medicine.disease 030104 developmental biology medicine.anatomical_structure Molecular Diagnostic Techniques 030220 oncology & carcinogenesis Mutation Molecular Medicine Female Test performance Detection rate business |
| Zdroj: | The Journal of Molecular Diagnostics. 18:407-415 |
| ISSN: | 1525-1578 |
| DOI: | 10.1016/j.jmoldx.2016.01.007 |
| Popis: | Detection of mutational alterations is important for guiding treatment decisions of lung non-small-cell carcinomas and thyroid nodules with atypical cytologic findings. Inoperable lung tumors requiring further testing for staging and thyroid lesions often are diagnosed using only cytology material. Molecular diagnostic tests of these samples typically are performed on cell blocks; however, insufficient cellularity of cell blocks is a limitation for test performance. In addition, some of the fixatives used while preparing cell blocks often introduces artifacts for mutation detection. Here, we applied qClamp xenonucleic technology and quantitative RT-PCR to cells microdissected directly from stained cytology smears to detect common alterations including mutations and translocations in non-small-cell carcinomas and thyroid lesions. By using this approach, we achieved a 1% molecular alteration detection rate from as few as 50 cells. Ultrasensitive methods of molecular alteration detection similar to the one described here will be increasingly important for the evaluation of molecular alterations in clinical scenarios when only tissue samples that are small are available. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|