Separase: a universal trigger for sister chromatid disjunction but not chromosome cycle progression
| Autor: | Nobuaki Kudo, Jan-Michael Peters, Germain M. Ducos, Nicole Firnberg, Karin G. Wirth, Anders Zetterberg, Chantal Desdouets, Gordana Wutz, Romeo Ricci, Kim Nasmyth, Janina Seznec, Shahryar Taghybeeglu |
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| Přispěvatelé: | Ricci, Romeo, Research Institute of Molecular Pathology (IMP), Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany], Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Karolinska Institute, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich) |
| Rok vydání: | 2006 |
| Předmět: |
Male
Chromosomal Proteins Non-Histone [SDV]Life Sciences [q-bio] Cell Cycle Proteins SACCHAROMYCES-CEREVISIAE Mice Chromosome Segregation 11 Medical and Health Sciences Research Articles Anaphase Mice Knockout Cell Cycle Nuclear Proteins DROSOPHILA SEPARASE Cell biology [SDV] Life Sciences [q-bio] Securin Establishment of sister chromatid cohesion METAPHASE COMPLEXES Female Chromatid Separase Life Sciences & Biomedicine COHESIN DNA Replication Timing Embryonic Development Mitosis FISSION YEAST Biology Article Cell Line Polyploidy Endopeptidases Animals Sister chromatids Science & Technology Cohesin CLEAVAGE Cell Biology 06 Biological Sciences Fibroblasts Hematopoietic Stem Cells Molecular biology ANAPHASE TRANSITION Liver Regeneration SPINDLE Hepatocytes Genes Lethal Carrier Proteins Developmental Biology |
| Zdroj: | The Journal of Cell Biology Journal of Cell Biology Journal of Cell Biology, 2006, 172 (6), pp.847-860. ⟨10.1083/jcb.200506119⟩ |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.200506119 |
| Popis: | International audience; Separase is a protease whose liberation from its inhibitory chaperone Securin triggers sister chromatid disjunction at anaphase onset in yeast by cleaving cohesin's kleisin subunit. We have created conditional knockout alleles of the mouse Separase and Securin genes. Deletion of both copies of Separase but not Securin causes embryonic lethality. Loss of Securin reduces Separase activity because deletion of just one copy of the Separase gene is lethal to embryos lacking Securin. In embryonic fibroblasts, Separase depletion blocks sister chromatid separation but does not prevent other aspects of mitosis, cytokinesis, or chromosome replication. Thus, fibroblasts lacking Separase become highly polyploid. Hepatocytes stimulated to proliferate in vivo by hepatectomy also become unusually large and polyploid in the absence of Separase but are able to regenerate functional livers. Separase depletion in bone marrow causes aplasia and the presumed death of hematopoietic cells other than erythrocytes. Destruction of sister chromatid cohesion by Separase may be a universal feature of mitosis in eukaryotic cells. |
| Databáze: | OpenAIRE |
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