Production of Functional Human Hemoglobin in Transgenic Swine
| Autor: | W Lago, M E Swanson, Michael J. Martin, V Huntress, Stephen H. Pilder, J K O'Donnell, John S. Logan, Carl A. Pinkert, C T Parsons, K Hoover |
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| Rok vydání: | 1992 |
| Předmět: |
Microinjections
Swine Zygote Transgene Biomedical Engineering Bioengineering Locus (genetics) Biology Applied Microbiology and Biotechnology Chromatography DEAE-Cellulose Animals Genetically Modified Hemoglobins Gene expression Animals Humans Alpha globulin Gene Locus control region Chromosome Mapping Molecular biology Globins Oxygen Regulatory sequence Molecular Medicine Hemoglobin Biotechnology |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/nbt0592-557 |
| Popis: | A construct containing the locus control region (LCR) from the human beta globin locus together with two copies of the human alpha 1 gene and a single copy of the human beta A gene was used to obtain three transgenic pigs. The transgenic pigs are healthy, not anemic, and grow at a rate comparable to non-transgenic littermates. All animals expressed the human genes. However, alpha globin was consistently expressed at higher levels than beta globin. Isolation of the human hemoglobin from both porcine hemoglobin and other non-hemoglobin proteins was accomplished by ion exchange chromatography. The purified porcine derived human hemoglobin exhibited an oxygen affinity similar to that of human derived human hemoglobin. |
| Databáze: | OpenAIRE |
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