Diff-Quik Cytologic Recognition of Chlamydophila psittaci in Orolabial Lesions of Stevens-Johnson Syndrome
Autor: | Cesar V. Reyes |
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Rok vydání: | 2010 |
Předmět: |
Male
Pathology medicine.medical_specialty Histology Adolescent Cytodiagnosis Azure Stains Sensitivity and Specificity Psittacosis Giemsa stain Pathology and Forensic Medicine law.invention law Ornithosis medicine Humans Chlamydia psittaci Chlamydophila Staining and Labeling biology business.industry Diff-Quik General Medicine medicine.disease biology.organism_classification Methylene Blue Gram staining Chlamydophila psittaci Xanthenes Doxycycline Stevens-Johnson Syndrome Phenazines Sputum Gentian Violet medicine.symptom Mouth Diseases business |
Zdroj: | Acta Cytologica. 54:692-694 |
ISSN: | 1938-2650 0001-5547 |
Popis: | Background Chlamydophila psittaci causes psittacosis, an ornithosis acquired usually from infected birds. The disease is often focal and pneumonic but on rare instances can be protean and fatal. Diagnosis is by Chlamydophila serology, which may take as long as 21 days or more. The recovery of the organisms from mice, eggs or tissue culture inoculated with the patient's blood or sputum is tedious and dangerous for laboratory personnel. On occasion, C psittaci inclusion bodies have also been detected in infected cells by fluorescent antibody, Giemsa or Gimenez staining. This report describes heretofore not previously reported recognition of the causative organisms in Diff-Quik―stained clinical cytologic materials. Case A 17-year-old man presented with fever and sore throat, associated with Steven-Johnson syndrome, of 6 days' duration. In the touch and scrape smears of the orolabial mucosal lesions, C psittaci inclusion bodies were recognizable in Diff-Quik―stained but not with Papanicolaou-stained smears and Gram stain. There were few to numerous organisms per macrophage, which were enlarged or bloated and usually collared by polymorphonuclear leukocytes. The diagnosis was supported by a therapeutic trial with doxycycline and confirmed by a positive third serological tests for C psittaci 3 weeks after discharge. Conclusion In a suspected or probable case of ornithosis, a rapid diagnosis of C psittaci inclusion bodies is possible in clinical cytology materials using Diff-Quik. |
Databáze: | OpenAIRE |
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